Abstract
In vivo electroporation works as an effective method to transfer exogenous genes into postnatal rodent forebrain. Nevertheless, two deficiencies were found in the reported methods. First, surgical operation brings unnecessary trauma to newborn pups. Second, the procedure was complicated and the transfection efficiency was relatively low. Here we improved the previous electroporation method and make it more simple and efficient. The pulse voltage was decreased to 90 v. DNA injection into one pup’s forebrain could be completed within 30 s without any surgical operation. More than 94% of injected neonates survived. Almost 100% of the survivors expressed the introduced gene and the expression persists as long as 20 days after injection. Thus, this method offers a powerful new way for gene function study in postnatal neurogenesis and neural development.
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Abbreviations
- d:
-
Days
- IIIV:
-
III ventricle
- VZ:
-
Ventricular zone
- LV:
-
Lateral ventricular
- PFA:
-
Para formaldehyde
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Acknowledgments
We thank Doctor Jun-Bo Huang for providing pEGFP-N1 plasmid. This work was supported by grants from National Nature Science Foundation of china (No.2011CB910800, 2012CB518200, 30871030) and Beijing Natural Science Foundation (No.5122033).
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Ding, XF., Zhao, YQ., Hu, ZY. et al. Efficient Gene Transfer into Neonatal Mouse Brain Using Electroporation. Neurochem Res 37, 1392–1398 (2012). https://doi.org/10.1007/s11064-012-0742-0
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DOI: https://doi.org/10.1007/s11064-012-0742-0