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Plasma Membrane-Associated Glycohydrolases Activation by Extracellular Acidification due to Proton Exchangers

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Abstract

In this paper, we show that the pH optimum for the plasma membrane (PM)-associated activity of four glycohydrolases (conduritol B epoxide sensitive β-glucosidase, β-glucosidase GBA2, β-hexosaminidase and β-galactosidase) measured on intact cells is acidic. Moreover, we show that drugs able to modify the efflux of protons across the PM, thus locally affecting the extracellular proton concentration close to the PM, are able to modulate the activities of these enzymes. These data strongly suggest that pH-dependent modulation of PM-associated glycohydrolases activities could be an effective way to locally modulate the cell surface glycoconjugate composition.

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Acknowledgments

This work was supported by grant PRIN (Italy) to S.S.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Dipartimento di Chimica, Biochimica e Biotecnologie per la Medicina, Università degli Studi di Milano, Via Fratelli Cervi 93, 20090, Segrate, Italy

    Massimo Aureli, Nicoletta Loberto, Rosaria Bassi, Anita Ferraretto, Silvia Perego, Patrizia Lanteri, Vanna Chigorno, Sandro Sonnino & Alessandro Prinetti

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  1. Massimo Aureli
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  2. Nicoletta Loberto
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  3. Rosaria Bassi
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Correspondence to Alessandro Prinetti.

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Special Issue: In Honor of Bob Leeden.

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Aureli, M., Loberto, N., Bassi, R. et al. Plasma Membrane-Associated Glycohydrolases Activation by Extracellular Acidification due to Proton Exchangers. Neurochem Res 37, 1296–1307 (2012). https://doi.org/10.1007/s11064-012-0725-1

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  • DOI: https://doi.org/10.1007/s11064-012-0725-1

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