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Plasma Membrane-Associated Glycohydrolases Along Differentiation of Murine Neural Stem Cells

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Abstract

The activities of plasma membrane associated sialidase Neu3, total β-glucosidase, CBE-sensitive β-glucosidase, non-lysosomal β-glucosyl ceramidase GBA2, β-galactosidase, β-hexosaminidase and sphingomyelinase were determined at three different stages of differentiation of murine neural stem cell cultures, corresponding to precursors, commited progenitors, and differentiated cells. Cell immunostaining for specific markers of the differentiation process, performed after 7 days in culture in presence of differentiating agents, clearly showed the presence of oligodendrocytes, astrocytes and neurons. Glial cells were the most abundant. Sialidase Neu3 after a decrease from progenitors to precursors, showed an increase parallel to the differentiation process. All the other glycosidases increased their activity along differentiation. The activity of CBE-sensitive β-glucosidase and GBA2 were very similar at the precursor stage, but CBE-sensitive β-glucosidase increased 7 times while GBA2 only two in the differentiated cells. In addition, we analysed also sphingomyelinase as enzyme specifically associated to sphingolipids. The activity of this enzyme increased from precursors to differentiated cells.

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Acknowledgments

This work was supported by grant PRIN (Italy) to S.S.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, Via Fratelli Cervi 93, 20090, Segrate, Italy

    Massimo Aureli, Rosaria Bassi, Nicoletta Loberto, Vanna Chigorno, Alessandro Prinetti & Sandro Sonnino

  2. Division of Regenerative Medicine, Stem Cells and Gene Therapy, San Raffaele Scientific Institute, San Raffaele Telethon Institute for Gene Therapy, Milan, Italy

    Angela Gritti & Alessandra Ricca

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  1. Massimo Aureli
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  2. Angela Gritti
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  3. Rosaria Bassi
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  6. Vanna Chigorno
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Correspondence to Sandro Sonnino.

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Special Issue: In Honour of Bob Leeden.

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Aureli, M., Gritti, A., Bassi, R. et al. Plasma Membrane-Associated Glycohydrolases Along Differentiation of Murine Neural Stem Cells. Neurochem Res 37, 1344–1354 (2012). https://doi.org/10.1007/s11064-012-0719-z

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  • DOI: https://doi.org/10.1007/s11064-012-0719-z

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