Amelioration of Age Associated Neuroinflammation on Long Term Bacosides Treatment


Bacopa monnieri (L.) is a revered medicinal plant of traditional Indian system of medicine effective against cognitive impairment in ageing and SDAT. In our previous study, long term administration of bacosides was found to exhibit remarkable anti ageing effect, ameliorate age associated neurochemical and neurobehavioral deficits and prevent hippocampal neuronal degeneration in middle aged and aged rat brain cortex. In continuation to the previous study, the present study aims to investigate the neuroprotective effect of bacosides against age related chronic neuroinflammation in Wistar rat brain on 3 months treatment. Recently, neuroinflammation has gained considerable interest in age associated neurodegeneration and pathologies like SDAT due to its slow onset and chronic nature. The results of the present study demonstrated the significant attenuation of age dependant elevation of pro inflammatory cytokines, iNOS protein expression, total nitrite and lipofuscin content in middle aged and aged rat brain cortex on long term oral administration of bacosides. Thus, the present results suggest that bacosides possess immense potential to act as a neuroprotective agent due to its pleiotropic action for the prevention of ageing complications and SDAT progression.

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  1. 1.

    WHO Report (2002) Active ageing: a policy framework, 2002 health report. World Health Organization, Geneva

    Google Scholar 

  2. 2.

    von Bernhardi R, Tichauer JE, Eugenin J (2010) Ageing dependant changes of microglial cells and their relevance for neurodegenerative disorders. J Neurochem 112:1099–1114

    Article  Google Scholar 

  3. 3.

    Franceschi C, Capri M, Monti D et al (2007) Inflammageing and anti-inflammageing: a systemic perspective on ageing and longevity emerged from studies in humans. Mech Ageing Dev 128:92–105

    PubMed  Article  CAS  Google Scholar 

  4. 4.

    Streit WJ, Sammons NW, Kuhns AJ et al (2004) Dystrophic microglia in the ageing human brain. Glia 45:208–212

    PubMed  Article  Google Scholar 

  5. 5.

    Holmes C, El-Okl M, Williams AL et al (2003) Systemic infection, interleukin 1beta, and cognitive decline in Alzheimer’s disease. J Neurol Neurosurg Psychiatry 74:788–789

    PubMed  Article  CAS  Google Scholar 

  6. 6.

    Tarkowski E, Andreasen N, Tarkowski A et al (2003) Intrathecal inflammation precedes development of Alzheimer’s disease. J Neurol Neurosurg Psychiatry 74:1200–1205

    PubMed  Article  CAS  Google Scholar 

  7. 7.

    Meda L, Baron P, Scarlato G (2001) Glial activation in Alzheimer’s disease: the role of Abeta and its associated proteins. Neurobiol Aging 22:885–893

    PubMed  Article  CAS  Google Scholar 

  8. 8.

    Dave UP, Chauvan V, Dalvi J (1993) Evaluation of BR-16 A (Mentat) in cognitive and behavioural dysfunction of mentally retarded children: a placebo-controlled study. Indian J Pediatr 60:423–428

    PubMed  Article  CAS  Google Scholar 

  9. 9.

    Roodenrys S, Booth D, Bulzoni S et al (2002) Chronic effects of Brahami (Bacopa monnieri) on human memory. Neuropsychopharmacology 27:279–281

    PubMed  Article  Google Scholar 

  10. 10.

    Bhattacharya SK, Bhattacharya A, Kumar A et al (2000) Antioxidant activity of Bacopa monniera in rat frontal cortex striatum and hippocampus. Phytother Res 14:174–179

    PubMed  Article  CAS  Google Scholar 

  11. 11.

    Bhattacharya SK, Kumar A, Ghosal S (1999) Effect of Bacopa monnieri on animal models of Alzheimer’s disease and perturbed central cholinergic markers of cognition in rats. In: Siva Sankar DV (ed) Molecular aspects of Asian medicines. PJD Publications, New York

    Google Scholar 

  12. 12.

    Russo A, Borrelli F, Campisi A et al (2003) Nitric oxide-related toxicity in cultured astrocytes: effect of Bacopa monnieri. Life Sci 73:1517–1526

    PubMed  Article  CAS  Google Scholar 

  13. 13.

    Russo A, Izzo AA, Borrelli F et al (2003) Free radical scavenging capacity and protective effect on DNA damage of Bacopa monniera L. Phytother Res 17:870–875

    PubMed  Article  Google Scholar 

  14. 14.

    Viji V, Helen A (2011) Inhibition of pro-inflammatory mediators: role of Bacopa monniera (L.) Wettst. Inflammopharmacol 19(5):283–291

    Article  CAS  Google Scholar 

  15. 15.

    Hostettmann K, Hostettmann M, Marston A (1991) Saponins. In: Charlwood BV, Banthorpe DV (eds) Terpenoids. Methods in plant biochemistry, vol 7. Academic Press, San Diego, pp 435–471

  16. 16.

    Gilchrist M, Savoie M, Nohara O et al (2002) Nitric oxide synthase and nitric oxide production in in vivo-derived mast cells. J Leukocyte Biol 71:618–624

    PubMed  CAS  Google Scholar 

  17. 17.

    Enesco HE, Kruk P (1981) Dietary restriction reduces fluorescent age pigment accumulation in rat. Exp Gerontol 4:357–361

    Article  Google Scholar 

  18. 18.

    Tappel AL, Fletcher B, Deamer B (1973) Effect of antioxidants and nutrients on lipid peroxidation fluorescent products and ageing parameters in the mouse. J Geront 28:415–424

    PubMed  CAS  Google Scholar 

  19. 19.

    Nolan Y, Maher FO, Martin DS et al. (2005) Role of interleukin-4 in regulation of age-related inflammatory changes in the hippocampus. J Biol Chem 280:9354

    Google Scholar 

  20. 20.

    Spaulding CC, Walford RL, Effros RB (1997) Calorie restriction inhibits the age-related dysregulation of the cytokines TNF-alpha and IL-6 in C3B10RF1 mice. Mech Ageing Dev 93:87–94

    PubMed  Article  CAS  Google Scholar 

  21. 21.

    Chung HS, Kang M, Cho C et al (2007) Inhibition of nitric oxide and tumour necrosis factor-a by Moutan cortex in activated mouse peritoneal macrophages. Biol Pharm Bull 30:912–916

    PubMed  Article  CAS  Google Scholar 

  22. 22.

    Vernet D, Bonavera JJ, Swerdloff RS et al (1998) Spontaneous expression of inducible nitric oxide synthase in the hypothalamus and other brain regions of ageing rats. Endocrinology 139(7):3254–3261

    PubMed  Article  CAS  Google Scholar 

  23. 23.

    Dawson VL, Dawson TM (1995) Physiological and toxicological actions of nitric oxide in the central nervous system. Adv Pharmacol 34:323–342

    PubMed  Article  CAS  Google Scholar 

  24. 24.

    Chalimoniuk M, Strosznajder J (1998) Aging modulates nitric oxide synthesis and cGMP levels in hippocampus and cerebellum. Effects of amyloid peptide. Mol Chem Neuropathol 35(1/3):77–95

    PubMed  Article  CAS  Google Scholar 

  25. 25.

    McCann SM (1997) The nitric oxide hypothesis of brain aging. Exp Gerontol 32:431–440

    PubMed  Article  CAS  Google Scholar 

  26. 26.

    Jyoti Amar, Sethi P, Sharma D (2007) Bacopa monniera prevents from aluminium neurotoxicity in the cerebral cortex of rat brain. J Ethnopharmacol 111:56–62

    PubMed  Article  Google Scholar 

  27. 27.

    Brunk UT, Terman A (2002) Lipofuscin: mechanisms of age-related accumulation and influence on cell functions. Free Radic Biol Med 33:611–619

    PubMed  Article  CAS  Google Scholar 

  28. 28.

    Eichhoff G, Busche MA, Garaschuk O (2008) In vivo calcium imageing of the ageing and diseased brain. Eur J Nucl Med Mol Imageing 35(1):S99–106

    Article  Google Scholar 

  29. 29.

    Xu H, Chen M, Manivannan A et al (2008) Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice. Ageing Cell 7:58–68

    Article  CAS  Google Scholar 

  30. 30.

    Fiala M, Lin J, Ringman J et al (2005) Ineffective phagocytosis of amyloid-beta by macrophages of Alzheimer’s disease patients. J Alzheimers Dis 7:221–232

    PubMed  CAS  Google Scholar 

  31. 31.

    Tan J, Town T, Crawford F et al (2002) Role of CD40 ligand in amyloidosis in transgenic Alzheimer’s mice. Nat Neurosci 5:1288

    PubMed  Article  CAS  Google Scholar 

  32. 32.

    McGeer PL, McGeer EG (2004) Inflammation and neurodegeneration in Parkinson’s disease. Parkinson Relat Disord 10(Suppl. 1):S3

    Article  Google Scholar 

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We acknowledge the Vice Chancellor, SASTRA University for granting permission to carry out this work in Centre for Advanced Research in Indian System of Medicine (CARISM).

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Correspondence to G. P. Dubey.

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Rastogi, M., Ojha, R.P., Devi, B.P. et al. Amelioration of Age Associated Neuroinflammation on Long Term Bacosides Treatment. Neurochem Res 37, 869–874 (2012).

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  • Ageing
  • SDAT
  • Inflammation
  • Lipofuscin
  • Bacosides