Skip to main content

Advertisement

SpringerLink
Log in

Neuroprotective Effect of Sesame Seed Oil in 6-Hydroxydopamine Induced Neurotoxicity in Mice Model: Cellular, Biochemical and Neurochemical Evidence

  • Original Paper
  • Published:
Neurochemical Research Aims and scope Submit manuscript

Abstract

Natural antioxidants have shown a remarkable reduction in oxidative stress due to excess formation of reactive oxygen species by enhancing antioxidant mechanism in the neurodegenerative disorders. Sesame seed oil (SO) is one of the most important edible oil in India as well as in Asian countries and has potent antioxidant properties thus the present study evaluated the neuroprotective effect of SO by using 6-Hydroxydopamine (6-OHDA)-induced Parkinson’s disease model in mice. The mice were fed an SO mix diet for 15 days and then 6-OHDA was injected into the right striatum of mice brain. Three weeks after 6-OHDA infusion, mice were sacrificed and the striatum was removed. The neuroprotective role of SO on the activities of antioxidant and non-antioxidant enzymes such as glutathione reductase (GR), glutathione-S-transferase (GST), glutathione peroxidase (GPx), catalase (CAT) and content of glutathione (GSH) and thiobarbituric acid reactive substance (TBARS) were studied in the striatum. The activities of all the above-mentioned enzymes decreased significantly in 6-OHDA group (Lesioned) when compared with Sham. The pretreatment of SO on antioxidant mechanism and dopamine level in the brain had shown some significant improvement in Lesion+SO (L+SO) group when compared with Lesioned group. However, NADPH oxidase subunit, Nox2 and inflammatory stimulator Cox2 expression was increased as well as antioxidant MnSOD level was decreased in Lesioned group while SO showed the inhibitory effect on the activation of Nox2 and Cox2 and restored MnSOD expression in L+SO group. Increased tyrosine hydroxylase (TH) expression in substantia nigra as well as dopamine and its metabolite DOPAC level in L+SO group also support our findings that SO may inhibit activation of NADPH oxidase dependent inflammatory mechanism due to 6-OHDA induced neurotoxicity in mice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Andersen JK (2004) Oxidative stress in neurodegeneration: cause or consequence? Nat Med 10:S18–S25

    Article  PubMed  Google Scholar 

  2. Cui K, Luo X, Xu K, Ven Murthy MR (2004) Role of oxidative stress in neurodegeneration: recent developments in assay methods for oxidative stress and nutraceutical antioxidants. Prog Neuropsychopharmacol Biol Psychiatry 28:771–799

    Article  PubMed  CAS  Google Scholar 

  3. Hara H, Hiramatsu H, Adachi T (2007) Pyrroloquinoline quinone is a potent neuroprotective nutrient against 6-hydroxydopamine-induced neurotoxicity. Neurochem Res 32:489–495

    Article  PubMed  CAS  Google Scholar 

  4. Chao J, Yu MS, Ho YS et al (2008) Dietary oxyresveratrol prevents parkinsonian mimetic 6-hydroxydopamine neurotoxicity. Free Radic Biol Med 45:1019–1026

    Article  PubMed  CAS  Google Scholar 

  5. Gao QG, Chen WF, Xie JX et al (2009) Ginsenoside Rg1 protects against 6- OHDA-induced neurotoxicity in neuroblastoma SK-N-SH cells via IGF-I receptor and estrogen receptor pathways. J Neurochem 109:1338–1347

    Article  PubMed  CAS  Google Scholar 

  6. Im HI, Nam E, Lee ES et al (2006) Baicalein Protects 6-OHDA -induced neuronal damage by suppressing oxidative stress. Korean J Physiol Pharmacol 10:309–315

    CAS  Google Scholar 

  7. Ahmad M, Saleem S, Ahmad AS, Yousuf S et al (2005) Ginkgo biloba affords dose-dependent protection against 6-hydroxydopamine-induced parkinsonism in rats:neurobehavioural, neurochemical and immunohistochemical evidences. J Neurochem 93:94–104

    Article  PubMed  CAS  Google Scholar 

  8. Sun AY, Wang Q, Simonyi A et al (2008) Botanical phenolics and brain health. Neuromol Med 10:259–274

    Article  CAS  Google Scholar 

  9. Zhao B (2009) Natural antioxidants protect neurons in Alzheimer’s disease and Parkinson’s disease. Neurochem Res 34:630–638

    Article  PubMed  CAS  Google Scholar 

  10. Shad KF, Al-Salam S, Hamza AA (2007) Sesame oil as a protective agent against doxorubicin induced cardio toxicity in rat. Am J Pharmacol Toxicol 2:159–163

    Article  CAS  Google Scholar 

  11. Ahmad S, Yousuf S, Ishrat T et al (2005) Effect of dietary sesame oil as antioxidant on brain hippocampus of rat in focal cerebral ischemia. Life Sci 79:1921–1928

    Article  Google Scholar 

  12. Jeng KC, Hou RC, Wang JC et al (2005) Sesamin inhibits lipopolysaccharide- induced cytokine production by suppression of p38 mitogen-activated protein kinase and nuclear factor-kappaB. Immunol Lett 97:101–106

    Article  PubMed  CAS  Google Scholar 

  13. Hsu DZ, Liu MY (2002) Sesame oil attenuates multiple organ failure and increases survival rate during endotoxemia in rats. Crit Care Med 30:1859–1862

    Article  PubMed  Google Scholar 

  14. Sankar D, Sambandam G, Ramakrishna Rao M et al (2005) Modulation of blood pressure, lipid profiles and redox status in hypertensive patients taking different edible oils. Clin Chim Acta 355:97–104

    Article  PubMed  CAS  Google Scholar 

  15. Cheng FC, Jinn TR, Hou RCW (2006) Neuroprotective effects of sesamin and sesamolin on gerbil brain in cerebral ischemia. Int J Biomed Sci 2:284–288

    CAS  Google Scholar 

  16. Lahaie-Collins V, Bournival J, Plouffe M et al (2008) Sesamin modulates tyrosine hydroxylase, superoxide dismutase, catalase, inducible NO synthase and interleukin-6 expression in dopaminergic cells under MPP+-induced oxidative stress. Oxid Med Cell Longev 1:54–62

    Article  PubMed  Google Scholar 

  17. Kong X, Yang JR, Guo LQ et al (2009) Sesamin improves endothelial dysfunction in renovascular hypertensive rats fed with a high-fat, high-sucrose diet. Eur J Pharmacol 620:84–89

    Article  PubMed  CAS  Google Scholar 

  18. Jeng KCG, Hou RCW (2005) Sesamin and sesamolin: nature’s therapeutic lignans. Curr Enzym Inhib 1:11–20

    Article  CAS  Google Scholar 

  19. Hamada N, Fujita Y, Tanaka A et al (2009) Metabolites of sesamin, a major lignan in sesame seeds, induce neuronal differentiation in PC12 cells through activation of ERK1/2 signaling pathway. J Neural Transm 116:841–852

    Article  PubMed  CAS  Google Scholar 

  20. Yokota T, Matsuzaki Y, Koyama M et al (2007) Sesamin, a lignan of sesame, down-regulates cyclin D1 protein expression in human tumor cells. Cancer Sci 98:1447–1453

    Article  PubMed  CAS  Google Scholar 

  21. Hou RC, Wu CC, Yang CH et al (2004) Protective effects of sesamin and sesamolin on murine BV-2 microglia cell line under hypoxia. Neurosci Lett 367:10–13

    Article  PubMed  CAS  Google Scholar 

  22. Joshi R, Kumar MS, Satyamoorthy K et al (2005) Free radical reactions and antioxidant activities of sesamol: pulse radiolytic and biochemical studies. J Agric Food Chem 53:2696–2703

    Article  PubMed  CAS  Google Scholar 

  23. Fazli IS, Abdin MZ, Jamal A et al (2005) Interactive effect of sulphur and nitrogen on lipid accumulation, acetyl- CoA concentration and acetyl-CoA carboxylase activity in developing seeds of oilseed crops (Brassica campestris L. and Eruca sativa Mill.). Plant Sci 168:29–36

    Article  CAS  Google Scholar 

  24. Budowski P, Connor RT, Field ET (1950) Sesameoil: IV. Determination of free and bound sesamol. J Am Oil Chem Soc 27:307–310

    Article  CAS  Google Scholar 

  25. Jollow DJ, Mitchell JR, Zampaghone N et al (1974) Bromobenzene induced oxide as the hepatotoxic intermediate. Pharmacology 11:161–169

    Article  Google Scholar 

  26. Utley HC, Bernhein F, Hochslein P (1967) Effects of sulfhydryl reagent on peroxidation in microsomes. Arch Biochem Biophys 260:521–531

    Google Scholar 

  27. Islam F, Zia S, Sayeed I et al (2002) Selenium induced alteration on lipids, lipid peroxidation, and thiol group in circadian rhythm centers of rat. Biol Trace Elem Res 90:1–12

    Article  Google Scholar 

  28. Habig WH, Pabst MJ, Jokoby WB (1974) Glutathione-S-transferase:the first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139

    PubMed  CAS  Google Scholar 

  29. Mohandas J, Marshall JJ, Duggin GG et al (1984) Differential distribution of glutathione and glutathione related enzymes in rabbit kidneys: possible implication in analgesic neuropathy. Cancer Res 44:5086–5091

    PubMed  CAS  Google Scholar 

  30. Carlberg I, Mannervik B (1975) Glutathione reductase levels in rat brain. J Biol Chem 250:5475–5480

    PubMed  CAS  Google Scholar 

  31. Caliborne A (1985) Catalase activity. In: Wakd G (ed) CRC hand book of methods for oxygen radical research. CRC Press, Boca Raton, pp 283–284

    Google Scholar 

  32. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Ann Biochem 44:276–287

    Article  CAS  Google Scholar 

  33. Bradford MM (1976) Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  34. Halliwell B (2006) Oxidative stress and neurodegeneration: where are we now? J Neurochem 97:1634–1658

    Article  PubMed  CAS  Google Scholar 

  35. Cheung YT, Lau WK, Yu MS et al (2009) Effects of all-trans-retinoic acid on human SH-SY5Y neuroblastoma as in vitro model in neurotoxicity research. Neurotox 30:127–135

    Article  CAS  Google Scholar 

  36. Wu JH, Hodgson JM, Clarke MW et al (2009) Inhibition of 20- hydroxyeicosatetraenoic acid synthesis using specific plant lignans: in vitro and human studies. Hyperten 54:1151–1158

    Article  CAS  Google Scholar 

  37. Fridovich I (1995) Superoxide radical and superoxide dismutases. Annu Rev Biochem 64:97–112

    Article  PubMed  CAS  Google Scholar 

  38. Boillée S, Cleveland DW (2008) Revisiting oxidative damage in ALS: microglia, Nox, and mutant SOD1. J Clin Invest 118:474–478

    PubMed  Google Scholar 

  39. Huang J, Philbert MA (1996) Cellular responses of cultured cerebellar astrocytes to ethacrynic acid-induced perturbation of subcellular glutathione homeostasis. Brain Res 711:184–192

    Article  PubMed  CAS  Google Scholar 

  40. Cruz-Auguado R, Almaguer-Melian W, Diaz MC (2001) Behavioural and biochemical effects of glutathione depletion in the rat brain. Brain Res Bull 55:327–333

    Article  Google Scholar 

  41. Baez S, Segura-Aguilar J, Widersten M (1997) Glutathione transferases catalyse the detoxication of oxidized metabolites (o-quinones) of catecholamines and may serve as an antioxidant system preventing degenerative cellular processes. Biochem J 324:25–28

    PubMed  CAS  Google Scholar 

  42. Halliwell B, Gutteridge JM, Cross C (1992) Free radicals, antioxidants, and human disease: where are we now? J Lab Clin Med 119:598–620

    PubMed  CAS  Google Scholar 

  43. Zafar KS, Siddiqui A, Sayeed I et al (2003) Dose-dependent protective effect of selenium in rat model of Parkinson’s disease: neurobehavioral and neurochemical evidences. J Neurochem 84:438–446

    Article  PubMed  CAS  Google Scholar 

  44. Khan MM, Ahmad A, Ishrat T et al (2010) Resveratrol attenuates 6- hydroxydopamine-induced oxidative damage and dopamine depletion in rat model of Parkinson’s disease. Brain Res 1328:139–151

    Article  PubMed  CAS  Google Scholar 

  45. Liu T, Jin H, Sun QR et al (2010) The neuroprotective effects of tanshinone IIA on β-amyloid-induced toxicity in rat cortical neurons. Neuropharmacol 59:595–604

    Article  CAS  Google Scholar 

  46. Brown DI, Griendling KK (2009) Nox proteins in signal transduction. Free Radic Biol Med 47:1239–1253

    Article  PubMed  CAS  Google Scholar 

  47. Nakano D, Kurumazuka D, Nagai Y et al (2008) Dietary sesamin suppresses aortic NADPH oxidase in DOCA salt hypertensive rats. Clin Exp Pharmacol Physiol 35:324–326

    Article  PubMed  CAS  Google Scholar 

  48. Anantharam V, Kaul S, Song C et al (2007) Pharmacological inhibition of neuronal NADPH oxidase protects against 1-methyl-4-phenylpyridinium (MPP+)-induced oxidative stress and apoptosis in mesencephalic dopaminergic neuronal cells. Neurotoxicology 28:988–997

    Article  PubMed  CAS  Google Scholar 

  49. Teismann P, Vila M, Choi DK (2003) COX-2 and neurodegeneration in Parkinson’s disease. Ann N Y Acad Sci 991:272–277

    Article  PubMed  CAS  Google Scholar 

  50. Cui Y, Hou X, Chen J et al (2010) Sesamin inhibits bacterial formylpeptide- induced inflammatory responses in a murine air-pouch model and inTHP-1 human monocytes. Nutr J 140:377–381

    Article  CAS  Google Scholar 

  51. Utsunomiya T, Chavali SR, Zhong WW et al (2000) Effects of sesamin- supplemented dietary fat emulsions on the ex vivo production of lipopolysaccharide-induced prostanoids and tumor necrosis factor alpha in rats. Am J Clin Nutr 72:804–808

    PubMed  CAS  Google Scholar 

  52. Jin F, Wu Q, Lu YF et al (2008) Neuroprotective effect of resveratrol on 6-OHDA-induced Parkinson’s disease in rats. Eur J Pharmacol 600:78–82

    Article  PubMed  CAS  Google Scholar 

  53. Callio J, Oury TD, Chu CT (2005) Manganese superoxide dismutase protects against 6-hydroxydopamine injury in mouse brains. J Biol Chem 280:18536–18542

    Article  PubMed  CAS  Google Scholar 

  54. Wang J, Du XX, Jiang H et al (2009) Curcumin attenuates 6-hydroxydopamine- induced cytotoxicity by anti-oxidation and nuclear factor-kappa B modulation in MES23.5 cells. Biochem Pharmacol 78:178–183

    Article  PubMed  CAS  Google Scholar 

  55. Rathore P, Dohare P, Varma S et al (2008) Curcuma oil: reduces early accumulation of oxidative product and is anti-apoptogenic in transient focal ischemia in rat brain. Neurochem Res 33:1672–1682

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

Authors are thankful to CSIR, Ministry of Science and Technology, New Delhi, Govt. of India for providing authentic sesame seed as a gift sample to carry out this research work. This work was supported by women young scientist award (Department of Science and Technology (DST), New Delhi, Govt. of India) to DPK. We greatly acknowledge Ms Sylvia Megyardi, Oral Biology and Anatomy, GHSU, Augusta, GA, USA for reviewing and editing this manuscript.

Conflict of interest

There is no conflict of interest with any person or other organization.

Author information

Author notes

  1. Saif Ahmad

    Present address: Department of Ophthalmology, School of Medicine, Georgia Health Sciences University (GHSU), Augusta, GA, 30912, USA

  2. M. Badruzzaman Khan

    Present address: Center for Molecular Chaperone/Radiobiology and Cancer Virology, School of Medicine, Georgia Health Sciences University (GHSU), Augusta, GA, 30912, USA

  3. Kanchan Bhatia

    Present address: Experimental Medicine, School of Medicine, Georgia Health Sciences University (GHSU), Augusta, GA, 30912, USA

  4. Rizwanul Haque

    Present address: Department of Microbiology and Immunology, C6749 Penn State College of Medicine, 500 University Drive, Hershey, PA, 17033, USA

  5. Inayat Saleem Fazili

    Present address: Center of Animal Biotechnology, Sher-i-Kashmir University of Agricultural Sciences and Technology, Shuhama (Alusteng), Srinagar, 190 006, India

  6. Arshad Jamal

    Present address: Biological Science Department, College of Science and Arts Rabigh, King Abdulaziz University, Jeddah, Saudi Arabia

  7. Deepshikha Pande Katare

    Present address: Amity Institute of Biotechnology, Amity University, Sector-125, Noida, Uttar Pradesh, 201303, India

  8. M. Nasrul Hoda

    Present address: Department of Neurology, School of Medicine, Georgia Health Sciences University (GHSU), Augusta, GA, 30912, USA

Authors and Affiliations

  1. Department of Biotechnology, Jamia Hamdard (Hamdard University), New Delhi, 110 062, India

    Saif Ahmad, Inayat Saleem Fazili, Arshad Jamal & Deepshikha Pande Katare

  2. Department of Medical Elementology and Toxicology, Faculty of Science, Jamia Hamdard (Hamdard University), New Delhi, 110062, India

    M. Badruzzaman Khan, M. Nasrul Hoda, Kanchan Bhatia & Rizwanul Haque

  3. Council of Scientific and Industrial Research(CSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110001, India

    Jafar Salamt Khan

Authors
  1. Saif Ahmad
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Badruzzaman Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Nasrul Hoda
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kanchan Bhatia
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rizwanul Haque
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Inayat Saleem Fazili
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Arshad Jamal
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jafar Salamt Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Deepshikha Pande Katare
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Saif Ahmad or Deepshikha Pande Katare.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ahmad, S., Khan, M.B., Hoda, M.N. et al. Neuroprotective Effect of Sesame Seed Oil in 6-Hydroxydopamine Induced Neurotoxicity in Mice Model: Cellular, Biochemical and Neurochemical Evidence. Neurochem Res 37, 516–526 (2012). https://doi.org/10.1007/s11064-011-0638-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11064-011-0638-4

Keywords

Search

Navigation