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Changes in TWIK-related Acid Sensitive K+-1 and -3 Channel Expressions from Neurons to Glia in the Hippocampus of Temporal Lobe Epilepsy Patients and Experimental Animal Model

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Abstract

In the present study, we analyzed expressions of tandem of P domains in a Weak Inwardly rectifying K+ channel (TWIK)-related Acid-Sensitive K+ (TASK) channel-1 and -3 in the hippocampus of patients with temporal lobe epilepsy (TLE) and in rat model. In the control human subjects, TASK-1, and -3 immunoreactivity was observed in pyramidal neurons and dentate granule cells. In TLE patients, TASK-1 and -3 immunoreactivity was rarely observed in neurons. However, TASK-1 immunoreactivity was observed in astrocytes, and TASK-3 immunoreactivity was detected in both astrocytes and microglia. In the rat hippocampus, TASK-1 immunoreactivity was observed in astrocytes within normal and epileptic hippocampus. The alterations in TASK-3 immunoreactivity in the rat hippocampus were similar to those in the human hippocampus. These findings reveal that TASK-1 and -3 are differentially expressed in the normal and epileptic hippocampus, and suggest that TASK channels may contribute to the properties of the epileptic hippocampus.

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Acknowledgments

This work was supported by National Research Foundation of Korea; Contract grant number: 2010K000808 and 2009-0093812.

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Author notes

  1. Ji-Eun Kim

    Present address: Department of Neurology, UCSF, and Veterans Affairs Medical Center, San Francisco, CA, 94121, USA

Authors and Affiliations

  1. Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chunchon, Kangwon-Do, 200-702, South Korea

    Ji-Eun Kim, Seong-Il Yeo, Hea Jin Ryu & Tae-Cheon Kang

  2. Institute of Epilepsy Research, College of Medicine, Hallym University, Chunchon, Kangwon-Do, 200-702, South Korea

    Ji-Eun Kim, Seong-Il Yeo, Hea Jin Ryu & Tae-Cheon Kang

  3. Department of Neurosurgery, College of Medicine, Seoul National University, Seoul, 110-744, South Korea

    Chun Kee Chung

  4. Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chunchon, Kangwon-Do, 200-702, South Korea

    Min-Ju Kim

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Correspondence to Tae-Cheon Kang.

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Kim, JE., Yeo, SI., Ryu, H.J. et al. Changes in TWIK-related Acid Sensitive K+-1 and -3 Channel Expressions from Neurons to Glia in the Hippocampus of Temporal Lobe Epilepsy Patients and Experimental Animal Model. Neurochem Res 36, 2155–2168 (2011). https://doi.org/10.1007/s11064-011-0540-0

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