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Doxycycline Attenuates Peripheral Inflammation in Rat Experimental Autoimmune Neuritis

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Abstract

Experimental autoimmune neuritis (EAN) is a T cell-mediated autoimmune inflammatory demyelinating disease of the peripheral nervous system and widely-used animal model of human inflammatory demyelinating polyradiculoneuropathies. Doxycycline is a well-known antibiotic and has been reported to have neuroprotective and anti-inflammatory effects. Here we investigated the effects of doxycycline on rat EAN. Therapeutic treatment with doxycycline (40 mg/kg body weight daily from the Day 9 to Day 14 post immunization) significantly attenuated the severity of EAN, decreased inflammatory infiltration of macrophages, B- and T-cells and demyelination in sciatic nerves of EAN rats. Pro-inflammatory molecules including matrixmetalloproteinase-9, inducible nitric oxide synthase and interleukin-17 were greatly decreased in sciatic nerves by administration of doxycycline as well. Taken together, our data showed that doxycycline could effectively suppress the peripheral inflammation to improve outcome of EAN, which suggests that doxycycline may be considered as a potential candidate of pharmacological treatment for neuropathies.

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Acknowledgments

Chenju Yi gratefully acknowledged China Scholarship Council. This investigation was partly supported by National Nature Science Foundation of China (No: 81070954).

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Authors and Affiliations

  1. Institute of Brain Research, University of Tuebingen, Calwer Street 3, 72076, Tuebingen, Germany

    Chenju Yi, Zhiyuan Zhang, Caroline Zug, Hermann J. Schluesener & Zhiren Zhang

  2. Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China

    Chenju Yi & Wei Wang

  3. Institute of Immunology, Third Military Medical University of PLA, Chongqing, People’s Republic of China

    Zhiren Zhang

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  1. Chenju Yi
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  2. Zhiyuan Zhang
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  3. Wei Wang
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  4. Caroline Zug
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Correspondence to Zhiren Zhang.

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C. Yi and Z. Zhang contributed equally to this work.

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Yi, C., Zhang, Z., Wang, W. et al. Doxycycline Attenuates Peripheral Inflammation in Rat Experimental Autoimmune Neuritis. Neurochem Res 36, 1984–1990 (2011). https://doi.org/10.1007/s11064-011-0522-2

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  • DOI: https://doi.org/10.1007/s11064-011-0522-2

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