Neurochemical Research

, Volume 36, Issue 11, pp 1947–1954 | Cite as

Stability Analysis of Vascular Endothelial Growth Factor in Cerebrospinal Fluid

  • Jun Yang
  • Stephen M. Dombrowski
  • Abhishek Deshpande
  • Natalie Krajcir
  • Serge El-Khoury
  • Chandra Krishnan
  • Mark G. LucianoEmail author
Original Paper


Vascular endothelial growth factor (VEGF) is a promising biological marker and prognostic indicator in many neurological diseases. Although VEGF concentrations in plasma and cerebrospinal fluid (CSF) are increasingly reported, CSF-VEGF stability pre- and during-assay procedures is seldom evaluated. In the current study, we investigated VEGF variability and stability in CSF related to sample preparation, storage, and routine experimental procedures. Results showed that contaminant cell breakdown or aggregation can occur gradually before sample processing. However, after the removal of contaminant cell components, CSF-VEGF levels did not show significant changes in samples incubated at room temperature for 5 h, thawed/refrozen for 6 cycles. Samples preserved at −80°C for up to 7 years continued to show measurable levels. Since some cellular components such as platelets contain a large amount of releasable VEGF, we conclude that CSF samples should be processed as soon as possible to carefully remove all cellular components and prevent possible consequent release of VEGF into CSF. After centrifugation to remove cellular contents, VEGF in CSF was relatively stable during routine experimental procedures and storage.


Cell count Centrifugation Temperature Time Preservation 



The authors would like to acknowledge the nursing staffs who work in the neurological ward (H60-61) at the Cleveland Clinic for their help in collecting samples and the team members who work in the Section of Pediatric and Congenital Neurological Surgery at the Cleveland Clinic for their help in clinical data collection.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Jun Yang
    • 1
  • Stephen M. Dombrowski
    • 1
  • Abhishek Deshpande
    • 1
  • Natalie Krajcir
    • 1
  • Serge El-Khoury
    • 1
  • Chandra Krishnan
    • 1
  • Mark G. Luciano
    • 1
    Email author
  1. 1.Department of Neurological Surgery, Section of Pediatric and Congenital Neurological Surgery, CSF Physiology Laboratory, Neurological InstituteCleveland ClinicClevelandUSA

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