Neurochemical Research

, Volume 35, Issue 12, pp 2144–2153 | Cite as

Modulation of PARP-1 and PARP-2 Expression by L-carnosine and Trehalose After LPS and INFγ-Induced Oxidative Stress

  • Vittoria Spina-Purrello
  • Salvatrice Giliberto
  • Vincenza Barresi
  • Vincenzo G. Nicoletti
  • Anna Maria Giuffrida Stella
  • Enrico Rizzarelli
ORIGINAL PAPER

Abstract

Poly(ADP-ribose) polymerases (PARPs) play a crucial role in DNA damage surveillance through their nick sensor functions. Since PARPs’ over activation leads to an excessive consumption of NAD+ and ATP depletion, these enzymes also are involved in the early events of programmed cell death as well as in necrosis. In order to verify the protective action of L-carnosine and trehalose against NO induced cell death, in the present study we examined their effects on the expression of PARP-1, PARP-2 and iNOS in primary rat astrocyte and oligodendrocyte cells, treated with lipopolysaccharide (LPS) and interferon gamma (INFγ), through semi-quantitative PCR and western analysis. To further characterize the molecular mechanisms underlying L-carnosine and trehalose action, we measured cell viability, nitrite production and LDH release. The data obtained clearly demonstrate that in the stress model employed L-carnosine and trehalose down regulate PARP-1 and PARP-2 expression in both cell phenotypes, thus suggesting their possible application in clinical trials.

Keywords

Astrocytes Oligodendrocytes PARP iNOS L-Carnosine Trehalose 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Vittoria Spina-Purrello
    • 1
  • Salvatrice Giliberto
    • 1
  • Vincenza Barresi
    • 1
  • Vincenzo G. Nicoletti
    • 1
  • Anna Maria Giuffrida Stella
    • 1
  • Enrico Rizzarelli
    • 2
    • 3
  1. 1.Department of Chemical Sciences, Section of Biochemistry and Molecular Biology, Medical FacultyUniversity of CataniaCataniaItaly
  2. 2.Department of Chemical SciencesUniversity of CataniaCataniaItaly
  3. 3.Istituto Biostrutture e Bioimmagini CNR Sez. di CataniaCataniaItaly

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