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Attenuation of Rotenone-Induced Mitochondrial Oxidative Damage and Neurotoxicty in Drosophila melanogaster Supplemented with Creatine

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Abstract

Creatine (Cr), an ergogenic nutritional supplement is demonstrated to possess bioenergetic, antiexcitotoxic and antioxidant properties. This study investigated the neuroprotective effects of Cr against rotenone induced oxidative stress, mortality and neurotoxicty in Drosophila melanogaster. We found significant diminution in the endogenous levels of oxidative markers in whole body homogenates of flies exposed to Cr (2–10 mM). Cr supplementation resulted in reduced mortality in flies exposed to rotenone (500 μM) and better performance in a negative geotaxis assay. Further Cr (10 mM) markedly offset rotenone induced mitochondrial oxidative stress, completely restored the GSH levels, nitric oxide levels, activity of Mn-SOD and dopamine depletion. In an oxidative stress bioassay, flies given Cr prophylaxis exhibited marked resistance to paraquat exposure. These data allow us to hypothesize that the neuroprotective action of Cr in Drosophila may be related to its direct antioxidant activity and ability to abrogate rotenone induced mitochondrial oxidative stress.

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Acknowledgments

We wish to thank the Director, CFTRI for his encouragement in this study. The first author (RKH) thanks the University Grant Commission (UGC), New Delhi, India for the award of Junior/Senior Research Fellowships.

Declaration of Interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the manuscript.

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Authors and Affiliations

  1. Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Council of Scientific and Industrial Research, Mysore, 570020, India

    Ravikumar Hosamani &  Muralidhara

  2. Department of Studies in Zoology, Manasagangotri, University of Mysore, Mysore, 570020, India

    Saraf R. Ramesh

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  1. Ravikumar Hosamani
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  3. Muralidhara
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Correspondence to Muralidhara.

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Hosamani, R., Ramesh, S.R. & Muralidhara Attenuation of Rotenone-Induced Mitochondrial Oxidative Damage and Neurotoxicty in Drosophila melanogaster Supplemented with Creatine. Neurochem Res 35, 1402–1412 (2010). https://doi.org/10.1007/s11064-010-0198-z

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