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Antioxidant and Electron Donating Function of Hypothalamic Polypeptides: Galarmin and Gx-NH2

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Abstract

Chemical mechanisms of antioxidant and electron donating function of the hypothalamic proline-rich polypeptides have been clarified on the molecular level. The antioxidant-chelating property of Galarmin and Gx-NH2 was established by their capability to inhibit copper(II) dichloride catalyzed H2O2 decomposition, thus preventing formation of HO• and HOO• radicals. The antiradical activity of Galarmin and Gx-NH2 was determined by their ability to react with 2,2-diphenyl-1-picrylhydrazyl radical applying differential pulse voltammetry and UV–Vis spectrophotometry methods. Galarmin manifest antiradical activity towards 2,2-diphenyl-1-picrylhydrazyl radical, depending on the existence of phenolic OH group in tyrosine residue at the end of the molecule. The presence of antiradical activity and reduction properties of Galarmin are confirmed by the existence of an oxidation specific peak in voltammograms made by differential pulse voltammetry at E ∘ = 0.795 V vs. Ag/Ag+ aq.

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Acknowledgments

This work has been implemented partially by the NFSAT EISI 01-01 grant support, for which we express our thanks.

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Authors and Affiliations

  1. Laboratory of Liquid Phase Free Radical Reactions, A.B.Nalbandyan Institute of Chemical Physics NAS RA, 5/2 Sevak str, Yerevan, 0014, Armenia

    L. A. Tavadyan, L. A. Harutunyan & H. G. Tonikyan

  2. Miller School of Medicine, University of Miami, 1600 NW 10th Ave, suite 8006 (r-2), Miami, FL, 33136, USA

    K. A. Galoian

  3. H.Kh. Buniatian Institute of Biochemistry, NAS RA, 5/1 Sevak str., Yerevan, 0014, Armenia

    A. A. Galoyan

Authors
  1. L. A. Tavadyan
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  2. K. A. Galoian
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  3. L. A. Harutunyan
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  4. H. G. Tonikyan
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  5. A. A. Galoyan
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Correspondence to L. A. Tavadyan.

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Special issue article in honour of Professor Armen Galoyan.

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Tavadyan, L.A., Galoian, K.A., Harutunyan, L.A. et al. Antioxidant and Electron Donating Function of Hypothalamic Polypeptides: Galarmin and Gx-NH2 . Neurochem Res 35, 947–952 (2010). https://doi.org/10.1007/s11064-010-0173-8

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  • DOI: https://doi.org/10.1007/s11064-010-0173-8

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