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Calcium Induces Expression of Cytoplasmic Gelsolin in SH-SY5Y and HEK-293 Cells

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Abstract

Gelsolin plays an important role in the regulation of amyloid beta-protein fibrillogenesis. We report here that calcium ionophore A23187 induces the expression of cytoplasmic gelsolin (c-gelsolin), and that protein kinase C (PKC) is involved in the up-regulation of c-gelsolin. In the presence of calcium, both SH-SY5Y and HEK-293 cells upon treatment with A23187 showed an increase in c-gelsolin expression in a concentration-dependent manner. Calcium-mediated up-regulation of c-gelsolin was inhibited by cycloheximide (a general inhibitor of protein synthesis). When cells were pretreated with staurosporine (an inhibitor of a variety of protein kinases including PKC), the up-regulation of c-gelsolin induced by A23187 was inhibited. Calphostin C, an inhibitor of PKC, blocked the up-regulation of c-gelsolin induced by A23187, while inhibitors of mitogen-activated protein kinases had no effect on c-gelsolin expression. In addition, phorbol-12-myristate-13-acetate, an activator of PKC, up-regulated c-gelsolin expression. These results suggest that calcium mediates up-regulation of c-gelsolin in a PKC-dependent manner.

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Abbreviations

Aβ:

Amyloid beta-protein

AD:

Alzheimer’s disease

APP:

β-amyloid precursor protein

CHX:

Cycloheximide

DAG:

Diacylglycerol

DMEM:

Dulbecco’s Modified Eagle’s Medium

DMSO:

Dimethylsulfoxide

ERK:

Extracellular signal-regulated kinase

IP3 :

Inositol 1,4,5-trisphosphate

JNK:

c-Jun N-terminal kinase

MAPKs:

Mitogen-activated protein kinases

PKC:

Protein kinase C

PLA2 :

Phospholipase A2

PLC:

Phospholipase C

PMA:

Phorbol-12-myristate-13-acetate

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Acknowledgments

This work was supported in part by funds from the New York State Office of Mental Retardation and Developmental Disabilities, and by NIH Grant No. AG020992.

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Authors and Affiliations

  1. Department of Neurochemistry, New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY, 10314, USA

    Lina Ji, Abha Chauhan & Ved Chauhan

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  1. Lina Ji
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  2. Abha Chauhan
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  3. Ved Chauhan
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Correspondence to Ved Chauhan.

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Ji, L., Chauhan, A. & Chauhan, V. Calcium Induces Expression of Cytoplasmic Gelsolin in SH-SY5Y and HEK-293 Cells. Neurochem Res 35, 1075–1082 (2010). https://doi.org/10.1007/s11064-010-0157-8

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