Abstract
The effect of fluoride exposure during gestation and post gestation periods were studied to check the status of oxidant, antioxidant and macromolecular changes in CNS and ameliorative role of antioxidants. The pregnant Wistar albino rats were exposed to 50 and 150 ppm fluoride in drinking water and the pups born to them were used for experimentation. After postpartum, the pups were administered daily selected antioxidants through oral gavage. On 21st postnatal day pups were sacrificed and biochemical parameters were assessed. Fluoride exposure substantially increased the activity/levels of fluoride, LPO, protein oxidation, MAO-B, GOT, GPT and decreased protein thiols, RNA and total proteins in discrete regions of CNS. The findings evidenced fluoride induced dyshomeostasis caused on antioxidants, enzymes, macromolecules and governed the pathophysiological events leading to functional loss in a dose dependent manner. The administration of antioxidants remedied the disquiet caused by high fluoride exposure at extreme vulnerable periods of life.
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Ozsvath DL (2009) Fluoride and environmental health: a review. Rev Environ Sci Biotechnol 8:59–79
Armstrong WD, Singer L, Makowski EL (1970) Placental transfer of fluoride and calcium. Am J Obstet Gynecol 107:432–434
Gupta S, Seth AK, Gupta A, Gavane AG (1993) Transplacental passage of fluorides. J Pediatr 123:139–141
Varner JA, Jensen KF, Horvath W, Isaacson RL (1998) Chronic administration of aluminum-fluoride or sodium-fluoride to rats in drinking water: alterations in neuronal and cerebrovascular integrity. Brain Res 784:284–298
Inkielewicz I, Czanowski W (2008) Oxidative stress parameters in rats exposed to fluoride and aspirin. Fluoride 41:76–82
Berlett BS, Stadtman ER (1997) Protein oxidation in aging, disease, and oxidative stress. J Biol Chem 272:20313–20316
Butterfield DA (2006) Oxidative stress in neurodegenerative disorders. Antioxid Redox Signal 8:1971–1973
Donnelly DF, Carroll JL (2005) Mitochondrial function and carotid body transduction. High Alt Med Biol 6:121–132
Pansarasa O, Castagna L, Colombi B, Vecchiet J, Felzani G, Marzatico F (2000) Age and sex differences in human skeletal muscle: role of reactive oxygen species. Free Radic Res 33:287–293
Tilson HA (1998) Developmental neurotoxicology of endocrine disruptors and pesticides: identification of information gaps and research needs. Environ Health Perspect 106(Suppl 3):807–811
Marzani B, Balage M, Venien A, Astruc T, Papet I, Dardevet D, Mosoni L (2008) Antioxidant supplementation restores defective leucine stimulation of protein synthesis in skeletal muscle from old rats. J Nutr 138:2205–2211
Mecocci P, Mariani E, Cornacchiola V, Polidori MC (2004) Antioxidants for the treatment of mild cognitive impairment. Neurol Res 26:598–602
Susheela AK, Bhatnagar M (2002) Reversal of fluoride induced cell injury through elimination of fluoride and consumption of diet rich in essential nutrients and antioxidants. Mol Cell Biochem 234–235:335–340
Chinoy NJ, Memon MR (2001) Beneficial effects of some vitamins and calcium on fluoride and aluminium toxicity on gastrocnemius muscle and liver of male mice. Fluoride 34:21–33
Madhusudhan N, Basha PM, Begum S, Ahmed F (2009) Fluoride induced neuronal oxidative stress and its amelioration by antioxidants in developing rats. Fluoride 42:179–187
Inkielewicz I, Czarnowski W, Krechniak J (2003) Determination of fluoride in soft tissues. Fluoride 36:16–20
Niehaus WG Jr, Samuelsson B (1968) Formation of malonaldehyde from phospholipid arachidonate during microsomal lipid peroxidation. Eur J Biochem 6:126–130
Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadtman ER (1990) Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 186:464–478
Reznick AZ, Packer L (1994) Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol 233:357–363
Green AL, Haughton TM (1961) A colorimetric method for the estimation of monoamine oxidase. Biochem J 78:172–175
Reitman S, Frankel S (1957) A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28:56–63
Munro HN (1966) The determination of nucleic acids. Methods Biochem Anal 14:113–176
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Mullenix PJ, Denbesten PK, Schunior A, Kernan WJ (1995) Neurotoxicity of sodium fluoride in rats. Neurotoxicol Teratol 17:169–177
Narayanaswamy M, Piler MB (2010) Effect of maternal exposure of fluoride on biometals and oxidative stress parameters in developing CNS of rat. Biol Trace Elem Res 133:71–82
Lieber CS, DeCarli LM (1991) Hepatotoxicity of ethanol. J Hepatol 12:394–401
Salido GM (2009) Oxidative stress, intracellular calcium signals and apoptotic processes. In: Salido GM, Rosado JA (eds) Apoptosis: involvement of oxidative stress and intracellular Ca2+ Homeostasis. Springer, Berlin, pp 1–16
Stadtman ER, Berlett BS (1997) Reactive oxygen-mediated protein oxidation in aging and disease. Chem Res Toxicol 10:485–494
Iciek M, Chwatko G, Lorenc-Koci E, Bald E, Wlodek L (2004) Plasma levels of total, free and protein bound thiols as well as sulfane sulfur in different age groups of rats. Acta Biochim Pol 51:815–824
Butterfield DA, Sultana R (2008) Redox proteomics: understanding oxidative stress in the progression of age-related neurodegenerative disorders. Expert Rev Proteomics 5:157–160
Murali G, Panneerselvam C (2007) Age-associated oxidative macromolecular damages in rat brain regions: role of glutathione monoester. J Gerontol A Biol Sci Med Sci 62:824–830
Cadenas E, Davies KJ (2000) Mitochondrial free radical generation, oxidative stress, and aging. Free Radic Biol Med 29:222–230
Nayak P, Chatterjee AK (2002) Response of regional brain glutamate transaminases of rat to aluminum in protein malnutrition. BMC Neurosci 3:12
Moss DW, Henderson AR (1999) Clinical enzymology. Harcourt Brace and Company Asia Pvt. Ltd., Singapore
Dean RT, Fu S, Stocker R, Davies MJ (1997) Biochemistry and pathology of radical-mediated protein oxidation. Biochem J 324(Pt 1):1–18
Grune T, Merker K, Sandig G, Davies KJ (2003) Selective degradation of oxidatively modified protein substrates by the proteasome. Biochem Biophys Res Commun 305:709–718
Leong SF, Lai JC, Lim L, Clark JB (1984) The activities of some energy-metabolising enzymes in nonsynaptic (free) and synaptic mitochondria derived from selected brain regions. J Neurochem 42:1306–1312
Guney M, Oral B, Take G, Giray SG, Mungan T (2007) Effect of fluoride intoxication on endometrial apoptosis and lipid peroxidation in rats: role of vitamins E and C. Toxicology 231:215–223
Padayatty SJ, Katz A, Wang Y, Eck P, Kwon O, Lee JH, Chen S, Corpe C, Dutta A, Dutta SK, Levine M (2003) Vitamin C as an antioxidant: evaluation of its role in disease prevention. J Am Coll Nutr 22:18–35
Ortwerth BJ, Prabhakaram M, Nagaraj RH, Linetsky M (1997) The relative UV sensitizer activity of purified advanced glycation endproducts. Photochem Photobiol 65:666–672
Dreher D, Junod AF (1996) Role of oxygen free radicals in cancer development. Eur J Cancer 32A:30–38
Kojo S (2004) Vitamin C: basic metabolism and its function as an index of oxidative stress. Curr Med Chem 11:1041–1064
Bray TM, Bettger WJ (1990) The physiological role of zinc as an antioxidant. Free Radic Biol Med 8:281–291
Noseworthy MD, Bray TM (2000) Zinc deficiency exacerbates loss in blood-brain barrier integrity induced by hyperoxia measured by dynamic MRI. Proc Soc Exp Biol Med 223:175–182
Flora SJ, Jain VK, Behari JR, Tandon SK (1982) Protective role of trace metals in lead intoxication. Toxicol Lett 13:51–56
Leibovitz BE, Siegel BV (1980) Aspects of free radical reactions in biological systems: aging. J Gerontol 35:45–56
Tappel ME, Chaudiere J, Tappel AL (1982) Glutathione peroxidase activities of animal tissues. Comp Biochem Physiol B 73:945–949
Beckett GJ, Arthur JR (2005) Selenium and endocrine systems. J Endocrinol 184:455–465
Acknowledgments
University Grants Commission, New Delhi, India for project grants F.No. 31-220/2005 (SR) dated 31-3-2006.
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Basha, P.M., Madhusudhan, N. Pre and Post Natal Exposure of Fluoride Induced Oxidative Macromolecular Alterations in Developing Central Nervous System of Rat and Amelioration by Antioxidants. Neurochem Res 35, 1017–1028 (2010). https://doi.org/10.1007/s11064-010-0150-2
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DOI: https://doi.org/10.1007/s11064-010-0150-2