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An Electron Spin Resonance Study for Real-time Detection of Ascorbyl Free Radicals After Addition of Dimethyl Sulfoxide in Murine Hippocampus or Plasma During Kainic Acid-Induced Seizures

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Abstract

Electron spin resonance (ESR)-silent ascorbate solutions generate a detectable, likely concentration-dependent signal of ascorbyl free radicals (AFR) immediately upon addition of a molar excess of dimethyl sulfoxide (DMSO). We aimed to perform quantitative ESR analysis of AFR in real time after addition of DMSO (AFR/DMSO) to evaluate ascorbate concentrations in fresh hippocampus or plasma following systemic administration of kainate in mice. Use of a special tissue-type quartz cell allowed immediate detection of AFR/DMSO ESR spectra in fresh tissues from mice. AFR/DMSO content was increased significantly in fresh hippocampus or plasma obtained during kainate-induced seizures of mice, reaching maximum levels at 90 min after intraperitoneal administration of 50 mg/kg kainic acid. This suggests that oxidative injury of the hippocampus resulted from the accumulation of large amounts of ascorbic acid in the brain after kainic acid administration. AFR/DMSO content measured on an ESR spectrometer can be used for real-time evaluation of ascorbate content in fresh tissue. Due to the simplicity, good performance, low cost and real-time monitoring of ascorbate, this method may be applied to clinical research and treatment in the future.

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We confirm that we have read the Journal’s position on issues involved in ethical publication and affirm that this report is consistent with those guidelines.

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None of the authors has any conflict of interest to disclose.

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Authors and Affiliations

  1. Department of Anaesthesiology and Intensive Care Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi-Yufu, City-Oita, 879-5593, Japan

    Shigekiyo Matsumoto, Chihiro Shingu, Hironori Koga, Satoshi Hagiwara, Hideo Iwasaka & Takayuki Noguchi

  2. Department of Physiology, Oita University Faculty of Medicine, 1-1 Idaigaoka-Hasamamachi-Yufu, City-Oita, 879-5593, Japan

    Isao Yokoi

Authors
  1. Shigekiyo Matsumoto
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  2. Chihiro Shingu
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  3. Hironori Koga
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  4. Satoshi Hagiwara
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  5. Hideo Iwasaka
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  6. Takayuki Noguchi
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  7. Isao Yokoi
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Correspondence to Shigekiyo Matsumoto.

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Matsumoto, S., Shingu, C., Koga, H. et al. An Electron Spin Resonance Study for Real-time Detection of Ascorbyl Free Radicals After Addition of Dimethyl Sulfoxide in Murine Hippocampus or Plasma During Kainic Acid-Induced Seizures. Neurochem Res 35, 1010–1016 (2010). https://doi.org/10.1007/s11064-010-0148-9

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  • DOI: https://doi.org/10.1007/s11064-010-0148-9

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