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Reduction of Brain Infarction Induced by a Transient Brain Ischemia in mdr1a Knockout Mice

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Abstract

In order to evaluate the functional role of P-glycoprotein (P-gp) in cerebral ischemia, both multidrug resistance 1a knockout (KO) mice and wild-type mice were subjected to transient focal ischemia under a constant body and brain temperature about 37°C. The results showed that the volume of brain infarction induced by middle cerebral artery occlusion in KO mice was significantly smaller than that seen in wild-type mice, although there were no significant differences in cerebral blood flow, physiological data and on anatomical analysis of cerebrovasculature between both groups. We suggest that multidrug resistance 1a P-gp plays a role for adjusting the expressions of endogenous neuronal cell modulating substances, such as cytokines, neuronal peptides, and others, in the brain, which is consistent with a previous paper (Bobrov et al. Neurosci Lett 24: 6–11, 2008).

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Acknowledgments

This work was supported by Grant-in-Aid for Scientific Research (C) and (B) from the Japanese Ministry of Education, Culture, Sports, Science and Technology (no. 15591660: M. M. and no. 17390433: Y. W., respectively).

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Authors and Affiliations

  1. Department of Anesthesiology, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo, 160-0023, Japan

    Michihiro Murozono, Shohei Matsumoto, Daisuke Nagaoka & Atsushi Isshiki

  2. Department of Pathology, Tokyo Medical University, Shinjuku, Tokyo, 160-0023, Japan

    Shinya Okada

  3. Department of Pharmacology/Pharmacotherapy, School of Pharmaceutical Sciences, Nihon Pharmaceutical University, 1028 Komuro, Inamachi, Kitaadachi-gun, Saitama, 362-0806, Japan

    Yasuo Watanabe

Authors
  1. Michihiro Murozono
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  2. Shohei Matsumoto
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  3. Shinya Okada
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  4. Daisuke Nagaoka
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  5. Atsushi Isshiki
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Correspondence to Michihiro Murozono.

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Murozono, M., Matsumoto, S., Okada, S. et al. Reduction of Brain Infarction Induced by a Transient Brain Ischemia in mdr1a Knockout Mice. Neurochem Res 34, 1555–1561 (2009). https://doi.org/10.1007/s11064-009-9943-6

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  • DOI: https://doi.org/10.1007/s11064-009-9943-6

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