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Gene Expression Profiling of Rat Cerebral Cortex Development Using cDNA Microarrays

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Abstract

A large amount of genetic information is devoted to brain development. In this study, the cortical development in rats at eight developmental time points (four embryonic [E15, E16, E18, E20] and four postnatal [P0, P7, P14, P21]) was studied using a rat brain 10K cDNA microarray. Significant differential expression was observed in 467 of the 9,805 genes represented on the microarray. Two major Gene Ontology classes—cell differentiation and cell–cell signaling—were found to be important for cortical development. Genes for ribosomal proteins, heterogeneous nuclear ribonucleoproteins, and tubulin proteins were up-regulated in the embryonic stage, coincidently with extensive proliferation of neural precursor cells as the major component of the cerebral cortex. Genes related to neurogenesis, including neurite regeneration, neuron development, and synaptic transmission, were more active in adulthood, when the cerebral cortex reached maturity. The many developmentally modulated genes identified by this approach will facilitate further studies of cortical functions.

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Acknowledgments

The work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (NO. 2006-04424) and by the research fund of Hanyang University.

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  1. Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, 17 Haengdang-dong, Sungdong-gu, Seoul, 133-791, South Korea

    Ki-Hwan Lee, Dong-Hyun Yu & Yong-Sung Lee

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Correspondence to Yong-Sung Lee.

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Lee, KH., Yu, DH. & Lee, YS. Gene Expression Profiling of Rat Cerebral Cortex Development Using cDNA Microarrays. Neurochem Res 34, 1030–1038 (2009). https://doi.org/10.1007/s11064-008-9867-6

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