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Aβ(25–35) Attenuated SREBP Level in Nuclear Extracts of Serum-Deprived Human Neuroblastoma Cells

Neurochemical Research volume 34, Article number: 845 (2009) Cite this article

Abstract

Disturbance in cholesterol homeostasis appears to be an important factor in the pathogenesis of neurodegenerative disorders. The aim of the present study was to investigate sterol regulatory element binding protein (SREBP) levels in the nuclear extracts of human neuroblastoma cells and the possible interaction of β-amyloid peptide (Aβ) and cholesterol with this transcription factor. In this study, cultured human neuroblastoma cells (SHSY-5Y) were incubated in serum-deprived media in the presence or absence of Aβ(25–35) (1 μM) or cholesterol (300 μM) for 24 h. Nuclear extracts were subjected to SDS-PAGE, and SREBP cleavage product (68 kDa) was detected by immunoblotting. SREBP levels were elevated in the cells incubated 24 h in serum-deprived experimental media and were attenuated by Aβ or cholesterol-supplementation. It is likely that the ability of Aβ to release cholesterol into the medium and downregulate SREBP is due to a feedback mechanism.

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Acknowledgment

Support to PK from the Swedish Alzheimer Foundation is gratefully acknowledged.

Author information

Affiliations

  1. Department of Pharmacology, Faculty of Pharmacy, Hacettepe University, Sihhiye, Ankara, 06100, Turkey

    Pelin Kelicen & Mehtap Cincioğlu

  2. Department of Urology, Oncology Education and Research Hospital, Demetevler, Ankara, 06530, Turkey

    Fatih Hızlı

  3. Division of Molecular Neuropharmacology, Neurotec Department, Karolinska Institute, Karolinska University Hospital Huddinge, Novum 5th floor, 141 86, Stockholm, Sweden

    Agneta Nordberg

Authors
  1. Pelin Kelicen
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  2. Mehtap Cincioğlu
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  3. Fatih Hızlı
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  4. Agneta Nordberg
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Correspondence to Pelin Kelicen.

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Kelicen, P., Cincioğlu, M., Hızlı, F. et al. Aβ(25–35) Attenuated SREBP Level in Nuclear Extracts of Serum-Deprived Human Neuroblastoma Cells. Neurochem Res 34, 845 (2009). https://doi.org/10.1007/s11064-008-9832-4

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  • DOI: https://doi.org/10.1007/s11064-008-9832-4

Keywords

  • SREBP
  • Aβ
  • Cholesterol
  • Human neuroblastoma cells (SHSY-5Y)