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Dp71f Modulates GSK3-β Recruitment to the β1-Integrin Adhesion Complex

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Abstract

Previously, it was shown that Dp71f binds to the β1-integrin adhesion complex to modulate PC12 cell adhesion. The absence of Dp71f led to a failure in the β1-integrin adhesion complex formation. One of the structural proteins which links the β1-integrin cytoplasmic domain to the actin cytoskeleton is ILK. GSK3-β is an ILK substrate and the carboxi-terminal region of dystrophin 427 is a substrate for hierarchical phosphorylation by GSK3-β. Dp71f contains the carboxi-terminal domain present in dystrophin 427. By using co-immunoprecipitation assays, in the present work it is demonstrated that in the neuronal PC12 cell line an interaction between Dp71f and GSK3-β occurs. This interaction was corroborated by in vitro pulldown assays. We show that GSK3-β is recruited to the β1-integrin complex and that a reduced expression of Dp71f induces a reduced GSK3-β recruitment to the β1-integrin complex. In addition, the present work establishes that adhesion of PC12 cells to laminin does not influence the phosphorylation status of Dp71f.

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Acknowledgments

I would like to thank Victor Tapia Ramirez for his technical assistance. This work was supported by CONACyT-México.

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Authors and Affiliations

  1. University Center of Biomedical Research, Universidad de Colima, Avenida 25 de Julio 965 Col. Villa San Sebastián, C.P. 28045, Colima, Colima, Mexico

    Joel Cerna Cortés & Jesús Muñiz

  2. Faculty of Chemical Sciences, Universidad Veracruzana, Avenida Oriente 6 1009, A.P. 215, C.P. 94340, Orizaba, Veracruz, Mexico

    Eliud Alfredo Garcia Montalvo

  3. Department de Physiologie des Interactions (EA701), Institut de Biologie, Bvd Henry IV, 34060, Montpellier, France

    Dominique Mornet

  4. Department of Genetics and Molecular Biology, Centro de Investigación y Estudios Avanzados del IPN, Av. I.P.N. 2508 Col. San Pedro Zacatenco, C.P. 07360, Mexico, D.F., Mexico

    Efrain Garrido, Federico Centeno & Bulmaro Cisneros

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  1. Joel Cerna Cortés
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  2. Eliud Alfredo Garcia Montalvo
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Correspondence to Joel Cerna Cortés.

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Cortés, J.C., Montalvo, E.A.G., Muñiz, J. et al. Dp71f Modulates GSK3-β Recruitment to the β1-Integrin Adhesion Complex. Neurochem Res 34, 438–444 (2009). https://doi.org/10.1007/s11064-008-9802-x

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  • DOI: https://doi.org/10.1007/s11064-008-9802-x

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