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Anionic Selectivity Sequence of the Cl−–H+ Symporter in the Synaptosomal Preparation from Rat Brain Cortex

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Abstract

The Na+/H+ exchanger has been the only unequivocally demonstrated H+-transport mechanism in the synaptosomal preparation. We had previously suggested that a Cl−–H+ symporter (in its acidifying mode) is involved in cytosolic pH regulation in the synaptosomal preparation. Supporting this suggestion, we now show that: (1) when synaptosomes are transferred from PSS to either gluconate or sulfate solutions, the Fura-2 ratio remains stable instead of increasing as it does in 50 mM K solution. This indicates that these anions do not promote a plasma membrane depolarization. (2) Based in the recovery rate from the cytosolic alkalinization, the anionic selectivity of the Cl−–H+ symporter is NO −3  > Br− > Cl− >> I− = isethionate = sulfate = methanesulfonate = gluconate. (3) PCMB 10 μM inhibits the gluconate-dependent alkalinization by 30 ± 6%. (4) Neither Niflumic acid, 9AC, Bumetanide nor CCCP inhibits the recovery from the cytosolic alkalinization.

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Acknowledgments

This work was partially supported by CONACyT (México) 29212-N and UASLP FAI-C05-FAI-10-3.24 to SSA; CONACyT (México) 62220 to RET, UM, SSA; M.L. Torres was a recipient of a M.Sc. degree fellowship from CONACyT (México). We are indebted to Ulises Meza for his critical reading and to Peter Mandeville and Omar Sanchez-Armass for the style correction of the manuscript.

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  1. Departamento de Fisiología y Farmacología, Facultad de Medicina, Universidad Autónoma de San Luis Potosi, Av. V. Carranza 2405, San Luis Potosi, S.L.P., 78210, Mexico

    M. L. Torres, F. Ortega, I. Cuaranta, J. González & S. Sanchez-Armass

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  1. M. L. Torres
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  2. F. Ortega
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  3. I. Cuaranta
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  4. J. González
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  5. S. Sanchez-Armass
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Correspondence to S. Sanchez-Armass.

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Special issue article in honor of Dr. Ricardo Tapia.

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Torres, M.L., Ortega, F., Cuaranta, I. et al. Anionic Selectivity Sequence of the Cl−–H+ Symporter in the Synaptosomal Preparation from Rat Brain Cortex. Neurochem Res 33, 1574–1581 (2008). https://doi.org/10.1007/s11064-008-9685-x

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  • DOI: https://doi.org/10.1007/s11064-008-9685-x

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