Abstract
During hypoxia the respiratory network produces gasping in vivo and in vitro. To understand the mechanisms involved in such response and to validate in vitro findings, correlative studies are necessary. During perinatal age gasping generation is robust and then declines during postnatal development, possibly due to changes in either the rhythm generator (the pre-Bötzinger complex, PBC) and/or its motor outputs. We tested this hypothesis by recording respiratory response to hypoxia in vivo and in vitro during postnatal development. We found that postnatal age influences: (1) The hypoxia-induced pattern change in the PBC bursts, (2) The coupling between the PBC and the XII nucleus during prolonged hypoxia and (3) The ability of mice to gasp and autoresuscitate from hypoxic conditions. We conclude that the inability of mice to gasp during late postnatal development might be determined by a progressive uncoupling between the respiratory rhythm generator and its motor outputs in hypoxia.
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Acknowledgments
This study was supported by Grants from Conacyt 42870, 46161 and 59187. We would like to thank Josué Ramirez-Jarquín who contributed with some initial experiments. We also like to thank Miguel Angel Aguileta and Benito Ordaz for reviewing the manuscript and Juan Javier López Guerrero, Arturo Franco and José Rodolfo Fernández for technical support.
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Special issue article in honor of Dr. Ricardo Tapia.
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Peña, F., Meza-Andrade, R., Páez-Zayas, V. et al. Gasping Generation in Developing Swiss–Webster Mice In Vitro and In Vivo. Neurochem Res 33, 1492–1500 (2008). https://doi.org/10.1007/s11064-008-9616-x
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DOI: https://doi.org/10.1007/s11064-008-9616-x