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Protein Levels and Activity of Some Antioxidant Enzymes in Hippocampus of Subjects with Amnestic Mild Cognitive Impairment

Neurochemical Research volume 33, pages 2540–2546 (2008)Cite this article

Abstract

Mild cognitive impairment (MCI) is generally referred to the transitional zone between normal cognitive aging and early dementia or clinically probable Alzheimer’s disease (AD). Most individuals with amnestic MCI eventually develop AD, which suggests that MCI may be the earliest phase of AD. Oxidative stress is observed in brain from subjects with both AD and MCI. Among others, two possibilities for elevated oxidataive stress are decreased activity or elevated expression of antioxidant enzymes, the latter as a response to the former. Accordingly, in the current study, the protein levels and activity of some antioxidant enzymes in the hippocampus of control and MCI brain were measured using Western blot analysis and spectrophotometric methods, respectively. Alterations in the levels and activity of a number of antioxidant enzymes in MCI brain compared to age-matched controls were found. These results are consistent with the hypothesis that oxidative stress may be an early event in the progression of amnestic MCI to AD.

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Acknowledgements

The authors thank the faculty of the UK ADC for providing the brain specimens used for this study. This research was supported in part by NIH grants to D.A.B [AG-05119, AG-10836].

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Affiliations

  1. Department of Chemistry, Center of Membrane Sciences, and Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY, 40506-0055, USA

    Rukhsana Sultana, Marta Piroddi & D. Allan Butterfield

  2. Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY, 40506, USA

    Rukhsana Sultana & D. Allan Butterfield

  3. Department of Internal Medicine, Section of Applied Biochemistry, University of Perugia, Perugia, Italy

    Marta Piroddi & Francesco Galli

  4. Center of Membrane Sciences, University of Kentucky, Lexington, KY, 40506, USA

    D. Allan Butterfield

Authors
  1. Rukhsana Sultana
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  2. Marta Piroddi
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  3. Francesco Galli
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  4. D. Allan Butterfield
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Corresponding author

Correspondence to D. Allan Butterfield.

Additional information

Special issue article in honor of Dr. Anna Maria Giuffrida-Stella.

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Sultana, R., Piroddi, M., Galli, F. et al. Protein Levels and Activity of Some Antioxidant Enzymes in Hippocampus of Subjects with Amnestic Mild Cognitive Impairment. Neurochem Res 33, 2540–2546 (2008). https://doi.org/10.1007/s11064-008-9593-0

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  • DOI: https://doi.org/10.1007/s11064-008-9593-0

Keywords

  • Mild cognitive impairment
  • Oxidative stress
  • Hippocampus
  • Glutathione
  • Glucose-6-phosphate dehydrogenase
  • Glutathione reductase
  • Glutathione peroxidise
  • Superoxide dismutase