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Coordinated Expression of HuD and GAP-43 in Hippocampal Dentate Granule Cells During Developmental and Adult Plasticity

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Abstract

Previous work from our laboratory demonstrated that the RNA-binding protein HuD binds to and stabilizes the GAP-43 mRNA. In this study, we characterized the expression of HuD and GAP-43 mRNA in the hippocampus during two forms of neuronal plasticity. During post-natal development, maximal expression of both molecules was found at P5 and their levels steadily decreased thereafter. At P5, HuD was also present in the subventricular zone, where it co-localized with doublecortin. In the adult hippocampus, the basal levels of HuD and GAP-43 were lower than during development but were significantly increased in the dentate gyrus after seizures. The function of HuD in GAP-43 gene expression was confirmed using HuD-KO mice, in which the GAP-43 mRNA was significantly less stable than in wild type mice. Altogether, these results demonstrate that HuD plays a role in the post-transcriptional control of GAP-43 mRNA in dentate granule cells during developmental and adult plasticity.

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Acknowledgments

We dedicate this work to Dr. Naren Banik, in recognition to his outstanding achievements in multiple sclerosis and spinal cord injury research. This work was supported by NIH (NS30255) to NPB.

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  1. Daniel C. Tanner

    Present address: (DCT) Department of Biomedical Genetics, University of Rochester, 575 Elmwood Ave, Box 633, Rochester, NY, 14642, USA

Authors and Affiliations

  1. Department of Cell Biology and Physiology, University of New Mexico School of Medicine, 1 University of New Mexico—MSC08 4750, Albuquerque, NM, 87131, USA

    Federico Bolognani

  2. Department of Neurosciences, University of New Mexico School of Medicine, 915 Camino de Salud NE, Rm. 145, Albuquerque, NM, 87131, USA

    Daniel C. Tanner, Sayuri Nixon & Nora I. Perrone-Bizzozero

  3. Department of Physiology, Keio University School of Medicine, Tokyo, 160-8582, Japan

    Hirotaka J. Okano & Hideyuki Okano

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  1. Federico Bolognani
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  2. Daniel C. Tanner
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Correspondence to Federico Bolognani or Nora I. Perrone-Bizzozero.

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Special issue in honor of Naren Banik.

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Bolognani, F., Tanner, D.C., Nixon, S. et al. Coordinated Expression of HuD and GAP-43 in Hippocampal Dentate Granule Cells During Developmental and Adult Plasticity. Neurochem Res 32, 2142–2151 (2007). https://doi.org/10.1007/s11064-007-9388-8

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