Abstract
Prolyl oligopeptidase (POP) is a serine endoprotease that hydrolyses peptides shorter than 30-mer. POP may have a role in inositol 1,4,5-triphosphate (IP3) signaling and in the actions of antidepressants, and POP inhibitors have exhibited antiamnesic and neuroprotective properties. However, little is known about the distribution of POP protein in the brain. We used immunohistochemistry to localize POP enzyme in the human whole hemisphere and in the rat whole brain. In humans, the highest POP densities were observed in caudate nucleus and putamen, hippocampus and cortex. In the rat, the highest POP densities were found in substantia nigra, hippocampus, cerebellum and caudate putamen. In general, the distribution of POP in human and rat brains was very similar and resembled that of IP3 receptors. Our findings are support for a role of POP in movement regulation, cognition and possibly in IP3 signaling. The expression of POP in processing nuclei further supports its function beyond neuropeptide metabolism.
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Acknowledgments
These studies were supported by the Academy of Finland to Pekka T. Männistö (No. 210758/2004 and 117881/2006), TEKES (No. 221853) and Helsinki University Research Grants, and from Fundación Valenciana Centro de Investigación Príncipe Felipe (No. I-19) to J. Arturo García Horsman. J.A. García-Horsman is also supported by the program Ramón y Cajal from Ministerio de Educación y Ciencia (Spain). We wish to thank Mrs. Jaana Leskinen and Mrs. Pirjo Hänninen for their excellent technical assistance and Mr. Ewen MacDonald for his comments about the linguistic style of the paper.
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Dr. Erkki Tupala M.D. sadly passed away during the research project.
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Myöhänen, T.T., Venäläinen, J.I., Tupala, E. et al. Distribution of Immunoreactive Prolyl Oligopeptidase in Human and Rat Brain. Neurochem Res 32, 1365–1374 (2007). https://doi.org/10.1007/s11064-007-9316-y
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DOI: https://doi.org/10.1007/s11064-007-9316-y