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Effect of Resuscitation with 21% Oxygen and 100% Oxygen on NMDA Receptor Binding Characteristics Following Asphyxia in Newborn Piglets

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Abstract

The present study investigated the effect of reventilation with 21% and 100% oxygen following asphyxia in newborn piglets on NMDA receptor binding characteristics, Na+, K+-ATPase activity, and lipid peroxidation. After achieving a heart rate less than 60 beats per minute, asphyxiated piglets were reventilated with 21% oxygen or 100% oxygen. 3[H]MK-801 binding showed the Bmax in the 21% and 100% groups to be 1.53 ± 0.43 and 1.42 ± 0.35 pmol/mg protein (p = ns). Values for Kd were 4.56 ± 1.29 and 4.17 ± 1.05 nM (p = ns). Na+, K+-ATPase activity in the 21% and 100% groups were 23.5 ± 0.9 and 24.4 ± 3.9 μmol Pi/mg protein/h (p = ns). Conjugated dienes (0.05 ± 0.02 vs. 0.07 ± 0.03 μmol/g brain) and fluorescent compounds (0.54 ± 0.05 vs. 0.78 ±  0.19 μg quinine sulfate/g brain), were similar in both groups (p = ns). Though lipid peroxidation products trended higher in the 100% group, these data show that NMDA receptor binding and Na+, K+-ATPase activity were similar following reventilation with 21% or 100% oxygen after a single episode of mild asphyxia.

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Acknowledgments

I dedicate this work to the memory of E. Gregory Keating, Ph.D. The authors thank Ms. Joanna Kubin and Ms. AnLi Zhu for their expert technical support, and Karen Rudisill for assistance with manuscript preparation.

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Authors and Affiliations

  1. Department of Pediatrics, Drexel University College of Medicine, Philadelphia, PA, USA

    David Joseph Hoffman, Eric Lombardini, Om Prakash Mishra & Maria Delivoria-Papadopoulos

  2. St. Christopher’s Hospital for Children, Philadelphia, PA, USA

    David Joseph Hoffman, Eric Lombardini, Om Prakash Mishra & Maria Delivoria-Papadopoulos

  3. The Reading Hospital and Medical Center, Sixth Avenue and Spruce Street, West Reading, PA, 19612, USA

    David Joseph Hoffman

Authors
  1. David Joseph Hoffman
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  2. Eric Lombardini
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  3. Om Prakash Mishra
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  4. Maria Delivoria-Papadopoulos
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Corresponding author

Correspondence to David Joseph Hoffman.

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Funded in part by a grant from the American Academy of Pediatrics/American Heart Association/Neonatal Resuscitation Program and NIH grant number HD-20337.

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Hoffman, D.J., Lombardini, E., Mishra, O.P. et al. Effect of Resuscitation with 21% Oxygen and 100% Oxygen on NMDA Receptor Binding Characteristics Following Asphyxia in Newborn Piglets. Neurochem Res 32, 1322–1328 (2007). https://doi.org/10.1007/s11064-007-9307-z

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  • DOI: https://doi.org/10.1007/s11064-007-9307-z

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