Abstract
Quantum dots (QDs) are semiconductor nanocrystals emerging as a new class of fluorescent labels with large brightness, multi color fluorescence emission and resistance against photobleaching. Here we have used QDs as biological markers in an immunofluorescence approach.
In this work GABAA receptors of rat cerebellar granule cells have been studied and in particular we have visualized the β2/3 and δ subunits in live cells. The results obtained were compared to those gathered with conventional probes.
The images of the δ subunit in living cells appear to correspond to those expected for a subunit part of GABAA receptors mediating tonic inhibition in the granules cell bodies.
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Siano, S., Cupello, A., Pellistri, F. et al. A Quantum-Dot Nanocrystal Study of GABAA Receptor Subunits in Living Cerebellar Granule Cells in Culture. Neurochem Res 32, 1024–1027 (2007). https://doi.org/10.1007/s11064-006-9263-z
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DOI: https://doi.org/10.1007/s11064-006-9263-z