Neurochemical Research

, Volume 31, Issue 11, pp 1371–1374 | Cite as

Induction of Transcription Factor A-myb Expression in Reactive Astrocytes Following an Excitotoxic Lesion in the Mouse Hippocampus

  • Gye Sun Jeon
  • Hee Jine Byun
  • Sung Kyung Park
  • Sang Wook Park
  • Dong Woon Kim
  • Je Hoon Seo
  • Choong Ik Cha
  • Sa Sun Cho
Original Paper

Abstract

In the present study, we examined patterns of A-myb expression in the kainic acid (KA)-treated mouse hippocampus. Western blot analysis revealed that A-myb expression was dramatically increased in brain 3 days after KA treatment, and was sustained for more than 7 days. A-myb immunoreactivity was restricted to hippocampal neurons in control mice. Three days after KA treatment, strong A-myb immunoreactivity was observed in reactive astrocytes throughout the CA3 region. Thereafter, A-myb immunoreactive astrocytes gradually concentrated around the CA3 region in parallel with selective neuronal loss, and only a few A-myb immunoreactive astrocytes persisted in the CA3 region 14 days after KA treatment. These findings suggest that the A-myb plays a role in the reactive gliosis signaling pathway in KA-induced excitotoxic lesions.

Keywords

A-myb Reactive astrocytes Kainic acid Hippocampus 

References

  1. 1.
    Ness SA (1996) The Myb oncoprotein: regulating a regulator. Biochim Biophys Acta 1288:F123–139PubMedGoogle Scholar
  2. 2.
    Foos G, Grimm S, Klempnauer KH (1994) The chicken A-myb protein is a transcriptional activator. Oncogene 9:2481–2488PubMedGoogle Scholar
  3. 3.
    Trauth K, Mutschler B, Jenkins NA et al (1994) Mouse A-myb encodes a trans-activator and is expressed in mitotically active cells of the developing central nervous system, adult testis and B lymphocytes. EMBO J 13:5994–6005PubMedGoogle Scholar
  4. 4.
    Golay J, Erba E, Bernasconi S et al (1994) The A-myb gene is preferentially expressed in tonsillar CD38+, CD39-, and sIgM- B lymphocytes and in Burkitt’s lymphoma cell lines. J Immunol 153:543–553PubMedGoogle Scholar
  5. 5.
    Sleeman JP (1993) Xenopus A-myb is expressed during early spermatogenesis. Oncogene 8:1931–1941PubMedGoogle Scholar
  6. 6.
    Weston K, Bishop JM (1989) Transcriptional activation by the v-myb oncogene and its cellular progenitor, c-myb. Cell 58:85–93PubMedCrossRefGoogle Scholar
  7. 7.
    Sitzmann J, Noben-Trauth K, Klempnauer KH (1995) Expression of mouse c-myb during embryonic development. Oncogene 11:2273–2279PubMedGoogle Scholar
  8. 8.
    Jeon GS, Shin DH, Cho SS (2004) Induction of transcription factor c-myb expression in reactive astrocytes following intracerebroventricular kainic acid injection in mouse hippocampus. Neurosci Lett 360:13–16PubMedCrossRefGoogle Scholar
  9. 9.
    Cho J, Lee SH, Seo JH et al (2002) Increased expression of phosphatase and tensin homolog in reactive astrogliosis following intracerebroventricular kainic acid injection in mouse hippocampus. Neurosci Lett 334:131–134PubMedCrossRefGoogle Scholar
  10. 10.
    Laursen SE, Belknap JK (1986) Intracerebroventricular injections in mice. Some methodological refinements. J Pharmacol Methods 16:355–357PubMedCrossRefGoogle Scholar
  11. 11.
    Jeon GS, Park SW, Kim DW et al (2004) Glial expression of the 90-kDa heat shock protein (HSP90) and the 94-kDa glucose-regulated protein (GRP94) following an excitotoxic lesion in the mouse hippocampus. Glia 48:250–258PubMedCrossRefGoogle Scholar
  12. 12.
    Ness SA (2003) Myb protein specificity: evidence of a context-specific transcription factor code. Blood Cells Mol Dis 31:192–200PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Gye Sun Jeon
    • 1
  • Hee Jine Byun
    • 1
  • Sung Kyung Park
    • 1
  • Sang Wook Park
    • 1
  • Dong Woon Kim
    • 1
  • Je Hoon Seo
    • 2
  • Choong Ik Cha
    • 1
    • 3
  • Sa Sun Cho
    • 1
    • 3
  1. 1.Department of AnatomySeoul National University College of MedicineSeoulKorea
  2. 2.Department of AnatomyChungbuk National University College of MedicineCheongjuKorea
  3. 3.MRC Neuroscience Research InstituteSeoul National University College of MedicineSeoulKorea

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