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Acetylcholinesterase Activity in Rats Experimentally Demyelinated with Ethidium Bromide and Treated with Interferon Beta

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Abstract

The ethidium bromide (EB) demyelinating model was associated with interferon beta (IFN-β) to evaluate acetylcholinesterase (AChE) activity in the striatum (ST), hippocampus (HP), cerebral cortex (CC), cerebellum (CB), hypothalamus (HY), pons (PN) and synaptosomes from the CC. Rats were divided into four groups: I control (saline), II (IFN-β), III (EB) and IV (EB and IFN-β). After 7, 15 and 30 days rats (n = 6) were sacrificed, and the brain structures were removed for enzymatic assay. AChE activity was found to vary in all the brain structures in accordance with the day studied (7–15–30 days) (P < 0.05). In the group III, there was an inhibition of the AChE activity in the ST, CB, HY, HP and also in synaptosomes of the CC (P < 0.05). It was observed that IFN-β per se was capable to significantly inhibit (P < 0.05) AChE activity in the ST, HP, HY and synaptosomes of the CC. Our results suggest that one of the mechanisms of action of IFN-β is through the inhibition of AChE activity, and EB could be considered an inhibitor of AChE activity by interfering with cholinergic neurotransmission in the different brain regions.

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Acknowledgments

The authors wish to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, 141317/03-5), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS).

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Authors and Affiliations

  1. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcellos, 2600-Anexo, 90035-003, Porto Alegre, RS, Brazil

    C. M. Mazzanti & R. M. Spanevello

  2. Curso de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil

    L. B. Pereira, J. F. Gonçalves, R. Kaizer, M. Corrêa, M. Ahmed, V. M. Morsch & M. R. C. Schetinger

  3. Laboratório de Cirurgia Experimental, Centro de Ciências Rurais, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil

    A. Mazzanti & R. Festugatto

  4. Departamento de Patologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil

    D. L. Graça

Authors
  1. C. M. Mazzanti
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  2. R. M. Spanevello
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  3. L. B. Pereira
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  4. J. F. Gonçalves
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  5. R. Kaizer
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  6. M. Corrêa
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  7. M. Ahmed
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  8. A. Mazzanti
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  9. R. Festugatto
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  10. D. L. Graça
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  11. V. M. Morsch
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  12. M. R. C. Schetinger
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Corresponding author

Correspondence to M. R. C. Schetinger.

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Mazzanti, C.M., Spanevello, R.M., Pereira, L.B. et al. Acetylcholinesterase Activity in Rats Experimentally Demyelinated with Ethidium Bromide and Treated with Interferon Beta. Neurochem Res 31, 1027–1034 (2006). https://doi.org/10.1007/s11064-006-9112-0

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  • DOI: https://doi.org/10.1007/s11064-006-9112-0

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