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Activity of Lactate Dehydrogenase in Serum and Cerebral Cortex of Immature and Mature Rats after Hypobaric Hypoxia

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Abstract

In our previous studies we have found both an increase of lipid peroxidation damage (expressed as levels of thiobarbituric acid-reactive substances) in brain and plasma lactate concentration in 21-day-old rats after a 30-min exposure to hypobaric hypoxia. Pretreatment of rats with l-carnitine decreased both parameters. The aim of our present study was to determine if the l-carnitine-dependent decrease of plasma lactate could be due to a modification of lactate dehydrogenase (LDH) activity. We followed brain and blood serum LDH activity of 14-, 21- and 90-day-old Wistar rats. We found an increase of brain LDH activity with age. However, we did not observe any significant differences in LDH activity after exposure to hypobaric hypoxia or l-carnitine pretreatment. In contrast to brain, serum LDH activity did not show any clear age-dependence. The hypoxia exposure increased LDH activity of 21-day-old rats only. Pretreatment of rats with l-carnitine decreased serum LDH activity of 21- and 90-day-old rats probably due to membrane stabilizing role of l-carnitine. In conclusions, acute hypobaric hypoxia and/or l-carnitine pretreatment modified serum but not brain LDH activity.

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Acknowledgments

This study was partly supported by research grants no. 305/04/0500 from the Grant Agency of the Czech Republic and nos. 1M6798582302 and AV0Z 50110509 from Ministry of Education, Youth and Sports of the Czech Republic.

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Authors and Affiliations

  1. First Faculty of Medicine, Institute of Physiology, Charles University, 128 00, Prague 2, Czech Republic

    Jitka Koudelová

  2. Institute of Physiology, Academy of Science of the Czech Republic and Center for Cardiovascular Research, Vídeňská 1083, 142 20, Prague 4, Czech Republic

    Hana Rauchová & Martina Vokurková

Authors
  1. Jitka Koudelová
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  2. Hana Rauchová
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  3. Martina Vokurková
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Correspondence to Hana Rauchová.

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Koudelová, J., Rauchová, H. & Vokurková, M. Activity of Lactate Dehydrogenase in Serum and Cerebral Cortex of Immature and Mature Rats after Hypobaric Hypoxia . Neurochem Res 31, 915–919 (2006). https://doi.org/10.1007/s11064-006-9097-8

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