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Regulation of Norepinephrine Release from Isolated Bovine Irides by Histamine

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Abstract

In the present study, we investigated the effect of histamine on sympathetic neurotransmission from isolated, superfused bovine irides. We also studied the pharmacology of prejunctional histamine receptors that regulate the release of norepinephrine (NE) from this tissue. The effect of exogenous histamine and various histamine receptor agonists was examined on the release of [3H]-norepinephrine ([3H]NE) triggered by electrical field stimulation using the Superfusion Method. Histamine receptor agonists caused a concentration-dependent inhibition of field-stimulated [3H]NE overflow with the following rank order of potency: imetit > histamine > R-α-methylhistamine. In all cases, the inhibitory action of histamine receptor agonists was attenuated at high concentrations of these compounds. The histamine receptor antagonists, clobenpropit (H3-antagonist/H4-agonist) and thioperamide (H3-antagonist) blocked the inhibitory response elicited by R-α-methylhistamine and imetit, respectively. Inhibitory effects of R-α-methylhistamine and clonidine were not additive suggesting that prejunctional H3- and α2-adrenoceptors coexist at neurotransmitter release sites. We conclude that histamine produces an inhibitory action on sympathetic neurotransmission in the bovine iris, an effect mimicked by selective H3-receptor agonists and blocked by H3-antagonists.

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Acknowledgements

We acknowledge the excellent secretarial assistance of Ms. Carolyn Wahl of the University of Houston College of Pharmacy in preparing this manuscript. Furthermore, we are grateful to local slaughterhouses (J.F. O’Neill and Sons Company and Greater Omaha Packing Company) for their generous donation of cow eyeballs.

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Authors and Affiliations

  1. Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, 141 Science and Research Building 2, Houston, TX, 77204, USA

    Kaustubh H. Kulkarni & Sunny E. Ohia

  2. School of Pharmacy and Health Professions, Creighton University, 2500 California Plaza, Omaha, NE, 68178, USA

    Catherine A. Opere, Angela M. LeDay & Mohd A. Shara

Authors
  1. Kaustubh H. Kulkarni
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  2. Catherine A. Opere
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  3. Angela M. LeDay
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  4. Mohd A. Shara
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  5. Sunny E. Ohia
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Correspondence to Sunny E. Ohia.

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Kulkarni, K.H., Opere, C.A., LeDay, A.M. et al. Regulation of Norepinephrine Release from Isolated Bovine Irides by Histamine. Neurochem Res 31, 891–897 (2006). https://doi.org/10.1007/s11064-006-9093-z

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  • DOI: https://doi.org/10.1007/s11064-006-9093-z

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