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Measuring Brain Uptake and Incorporation into Brain Phosphatidylinositol of Plasma myo-[2H6]Inositol in Unanesthetized Rats: An Approach to Estimate In vivo Brain Phosphatidylinositol Turnover

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Abstract

The in vivo rate of turnover of phosphatidylinositol (PtdIns) in brain is not known. In brain, certain receptor-mediated signal transduction involves metabolism of PtdIns and a method to measure its turnover in awake animals is useful in studying the effect of lithium and other therapeutic agents. In a method described here, rats were infused subcutaneously with myo-[2H6]inositol (Ins*) using an osmotic pump and, at 1 and 8 weeks, concentrations of free myo-inositol (Ins) and Ins* in plasma and brain were measured by GC-MS (chemical ionization). Also, PtdIns and PtdIns* together in brain were isolated, and Ins and Ins* from their headgroups were released enzymatically and specific activity of incorporated inositol was measured. The specific activity of inositol reached a steady state in plasma within 1 week of infusion, but not in brain even at 8 weeks. However, in brain, the specific activity of phosphatidylinositol was same as that of inositol at both time-points, suggestive of fast turnover of PtdIns. The animal experiment and the analytical methodology described here should be useful for measuring the rate of turnover of brain PtdIns in pathological and drug treatment conditions.

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Acknowledgement

This research was entirely supported by the Intramural Research Program of the NIH.

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Authors and Affiliations

  1. Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, Bethesda, MD, 20892, USA

    Kaizong Ma, Joseph Deutsch, Nelly E. Villacreses, Thad A. Rosenberger, Stanley I. Rapoport & H. Umesha Shetty

  2. Molecular Imaging Branch, National Institute of Mental Health, National Institutes of Health, 10 Center Drive, Room B3C351, MSC 1003, Bethesda, MD, 20892, USA

    H. Umesha Shetty

Authors
  1. Kaizong Ma
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  2. Joseph Deutsch
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  3. Nelly E. Villacreses
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  4. Thad A. Rosenberger
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  5. Stanley I. Rapoport
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  6. H. Umesha Shetty
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Correspondence to H. Umesha Shetty.

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Ma, K., Deutsch, J., Villacreses, N.E. et al. Measuring Brain Uptake and Incorporation into Brain Phosphatidylinositol of Plasma myo-[2H6]Inositol in Unanesthetized Rats: An Approach to Estimate In vivo Brain Phosphatidylinositol Turnover. Neurochem Res 31, 759–765 (2006). https://doi.org/10.1007/s11064-006-9080-4

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  • DOI: https://doi.org/10.1007/s11064-006-9080-4

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