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Pretraining but not Preexposure to the Task Apparatus Prevents the Memory Impairment Induced by Blockade of Protein Synthesis, PKA or MAP Kinase in Rats

Neurochemical Research volume 30, pages 61–67 (2005)Cite this article

Abstract

Adult male Wistar rats were trained and tested in a step-down inhibitory avoidance task (0.4 mA footshock, 24 h training-test interval). Fifteen minutes before or 0, 1.5 or 3 hours after training, animals received a 0.8 μl intrahippocampal infusion of the protein synthesis inhibitor anisomycin (80 μg), the PKA inhibitor Rp-cAMP (0.05 μg), the MAPK kinase inhibitor PD 098059 (50 μM solution) or vehicle (phosphate buffer in saline, pH 7.4). Anisomycin, Rp-cAMP and PD 098059 impaired retention test performance in animals injected at different times, prior and after training. Pretraining with a low footshock intensity (0.2 mA) 24 h before training prevented the amnestic effect of all drugs studied. However, simple preexposure to the inhibitory avoidance apparatus did not alter the amnestic effects of all drugs. The results suggest that memory processing requires hippocampal mechanisms dependent on protein synthesis, PKA and MAPK kinase at different times after training. These findings suggest that weak training must be sufficient to produce some lasting cellular expression of the experience so that the enhancement of consolidation of a previously acquired memory is not dependent on protein synthesis, PKA or MAPK.

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Affiliations

  1. Laboratório de Neurociências, Universidade do Extremo Sul Catarinense, 88806-000, Criciúma, SC, Brazil

    João Quevedo & Marcio Rodrigo Martins

  2. Montreal Neurological Institute, McGill University, Montreal, Quebec, Canadá, H3A 2B4

    Monica R. M. Vianna

  3. Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, 90046-900, Porto Alegre, RS, Brazil

    Rafael Roesler

  4. Departamento de Bioquímica, Centro de Memória, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, 90035-003, Porto Alegre, RS, Brazil

    Fernanda de-Paris & Ivan Izquierdo

  5. Laboratório de Neurorreceptores, Instituto de Biologia Celular y Neurociência Eduardo de Robertis, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina

    Jorge H. Medina

  6. Laboratório de Neurociências, Universidade do Extremo Sul Catarinense, 88806-000, Criciúma, SC, Brazil

    João Quevedo

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  1. João Quevedo
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  2. Monica R. M. Vianna
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  3. Rafael Roesler
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  4. Marcio Rodrigo Martins
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  5. Fernanda de-Paris
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  6. Jorge H. Medina
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  7. Ivan Izquierdo
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Correspondence to João Quevedo.

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Quevedo, J., Vianna, M.R.M., Roesler, R. et al. Pretraining but not Preexposure to the Task Apparatus Prevents the Memory Impairment Induced by Blockade of Protein Synthesis, PKA or MAP Kinase in Rats. Neurochem Res 30, 61–67 (2005). https://doi.org/10.1007/s11064-004-9686-3

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Keywords

  • MAPK
  • memory
  • PKA
  • Protein synthesis
  • rat