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Behavior of Rats in the Open Field within the Early Period after Light-Degree Blast-Induced Neurotrauma

Blast-induced neurotrauma (BINT) is a specific type of traumatic brain injury (TBI). At present, this type of injury is rather widespread throughout the world due to its occurrence in military conflicts and terrorism acts. Consequences of such traumas form an important social and medical problem. Brain injuries in the case of BINT result mostly from a specific action of the main pathological factor of explosion, the shock wave. Experimental studies of BINT consequences have been concentrated on its different aspects and were carried out using variable techniques of modeling; thus, some links of the BINT pathogenesis have been insufficiently examined. In our study, we modeled BINT in rats using a self-made pneumatic device that produced a baroacoustic wave with the excess pressure of about 25 kPa and examined behavioral activity of the experimental animals subjected to the action of this factor in the open-field test within acute and early segments of the post-traumatic period. After initial increases in the intensities of horizontal and vertical motor activities (in the case of vertical activity, dramatic and more long-lasting), these behavioral phenomena were later on significantly suppressed. Exploration of openings in the arena floor (burrows) and the performance of grooming episodes were suppressed within the entire observation period. A part of grooming events was transformed into an abortive pattern. It should be concluded that high anxiety related to primary pathological effects of the shock wave were obvious within the acute period (days 1–7). Within the early post-traumatic period (days 7–21), the animals experienced fear and demonstrated signs of depression; this was probably related to secondary damage to the brain against the background of neuroinflammation and neuromediatory imbalance.

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References

  1. V. V. Suhorukov, L. P. Zabrodyna, and Ju. V. Bovt, “Blast mild traumatic injury: modern review,” East Eur. Sci. J., 57, No. 5, 4–8 (2020).

    Google Scholar 

  2. G. Uzunalli, S. Herr, A. M. Dieterly, et al., “Structural disruption of the blood-brain barrier in repetitive primary blast injury,” Fluids Barriers CNS, 1, No. 18, 2 (2021); https://doi.org/10.1186/s12987-020-00231-2.

  3. P. Arun, F. Rossetti, D. M. Wilder, et al., “Blast exposure leads to accelerated cellular senescence in the rat brain,” Front. Neurol., 11, 438 (2020); https://doi.org/10.3389/fneur.2020.00438.

    Article  PubMed  PubMed Central  Google Scholar 

  4. V. O. Korshniak, “The impact of the blast wave on the formation of neurological symptoms in patients with battle traumatic brain injury,” Int. Neurol. J., 83, No. 5, 83–87 (2016); https://doi.org/10.22141/2224-0713.5.83.2016.78475.

    Article  Google Scholar 

  5. C. L. Mac Donald, J. Barber, J. Patterson, et al., “Association between 5-year clinical outcome in patients with nonmedically evacuated mild blast traumatic brain injury and clinical measures collected within 7 days postinjury in combat,” JAMA Netw. Open, 2, No. 1, e186676 (2019); https://doi.org/10.1001/jamanetworkopen.2018.6676.

  6. M. T. Baker, J. C. Moring, W. J. Hale, et al., “Acute assessment of traumatic brain injury and post-traumatic stress after exposure to a deployment-related explosive blast,” Mil. Med., 183, No. 11–12, e555–e563 (2018); https://doi.org/10.1093/milmed/usy100.

    Article  PubMed  PubMed Central  Google Scholar 

  7. J. L. Sherman, L. J. Adams, C. F. Kutz, et al., “The challenge of managing patients suffering from TBI: The utility of multiparametric MRI,” CNS Spectrums, 26, No. 2, 178–179 (2021); https://doi.org/10.1017/S109285292000293X.

    Article  Google Scholar 

  8. Z. S. Bailey, W. B. Hubbard, and P. J. VandeVord, “Cellular mechanisms and behavioral outcomes in blast-induced neurotrauma: Comparing experimental setups,” Methods Mol. Biol. (Clifton, N.J.), 1462, 119–138 (2016); https://doi.org/10.1007/978-1-4939-3816-2_8.

  9. N. D. Osteraas and V. H. Lee, “Neuroradiology,” Handb. Clin. Neurol., 140, 49–65 (2017); https://doi.org/10.1016/B978-0-444-63600-3.00004-0.

    CAS  Article  PubMed  Google Scholar 

  10. D. L. Chin and J. E. Zeber, “Mental health outcomes among military service members after severe injury in combat and TBI,” Mil. Med., 185, No. 5–6, e711–e718 (2020); https://doi.org/10.1093/milmed/usz440.

    Article  PubMed  Google Scholar 

  11. Yu. V. Kozlova, Device for Studying the Effect of the Shock Wave of an Explosion on the Body, Utility model patent No. 146858 U, bul. No. 12, 24.03.2021.

  12. B. Cui, M. Wu, X. She, and H/ Liu, “Impulse noise exposure in rats causes cognitive deficits and changes in hippocampal neurotransmitter signaling and tau phosphorylation,” Brain Res., 1427, 35–43. (2012); https://doi.org/10.1016/j.brainres.2011.08.035.

  13. O. G. Rodinskii, Yu. V. Kozlova, S. V. Kozlov, et al., “Doxorubicin-induced cardiomyopathy in rats: behavior of the animals in the open field,” Neurophysiology, 50, No. 4, 259–264 (2018); https://doi.org/10.1007/s11062-018-9747-x.

    CAS  Article  Google Scholar 

  14. Yu. N. Vovk and O. Yu. Vovk, Guide to Clinical Anatomy with Fundamentals of Surgeons. Part I, Kharkiv-Rubizhne, 346 (2017).

  15. J. Goeller, А. Wardlaw, D. Treichler, et al., “Investigation of cavitation as a possible damage mechanism in blast-induced traumatic brain injury,” J. Neurotrauma, 29, No. 10, 1970–1981 (2012); https://doi.org/10.1089/neu.2011.2224.

    Article  PubMed  Google Scholar 

  16. A. Nakagawa, G. T. Manley, A. D. Gean, et al., “Mechanisms of primary blast-induced traumatic brain injury: insights from shock-wave research”, J. Neurotrauma, 28, No. 6, 1101–1119 (2011); https://doi.org/10.1089/neu.2010.1442.

    Article  PubMed  Google Scholar 

  17. G. Polich, M. A. Iaccarino, and R. Zafonte, “Chapter 15 – Psychopharmacology of traumatic brain injury”, Handb. Clin. Neurol., 165, 253–267 (2019); https://doi.org/10.1016/B978-0-444-64012-3.00015-0.

  18. M. Alsalem, M. Haddad, A. Altarifi, et al., “Impairment in locomotor activity as an objective measure of pain and analgesia in a rat model of osteoarthritis,” Exp. Ther. Med., 20, No. 6, 165 (2020); https://doi.org/10.3892/etm.2020.9294.

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Kozlova, Y.V. Behavior of Rats in the Open Field within the Early Period after Light-Degree Blast-Induced Neurotrauma. Neurophysiology 53, 101–108 (2022). https://doi.org/10.1007/s11062-022-09921-z

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Keywords

  • blast-induced neurotrauma (BINT)
  • open field
  • motor activity
  • orientational/research activity
  • emotional state