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Effects of Citicoline on Structural/Functional Consequences of Focal Ischemia of the Rat Brain

Effects of citicoline on the volume of ischemically damaged cerebral tissue, dynamics of neurological deficiency, and indices of neurobehavioral tests were examined in rats with experimental transient focal cerebral ischemia induced by 60-min-long occlusion of the middle cerebral artery (MCA). Citicoline was administered immediately after beginning of reperfusion, and introductions were repeated for seven days. It was found that, on the 7th day after ischemia/reperfusion, the volume of the ischemic infarction (significantly damaged cerebral tissue) in rats with no citicoline treatment was, on average, 159.6±20.5 mm3, while in citicoline-treated rats the respective value was 86.5±10.8 mm3 (P < 0.01). In control untreated “infarction” rats, indices of the neurological deficiency demonstrated a very weak improvement within the observation period (5.9±1.0; 5.6±0.5, and 5.0±1.0 points according to the McGraw scale on the 1st, 3rd, and 7th days, respectively). Treatment with citicoline provided smaller intensities of the neurological deficiency throughout the period of observation (4.4±0.5; 3.5±1.1, and 1.9±0.5 points, respectively). The open-field and beam-walk tests demonstrated the positive effects of citicoline on locomotor and orientational/research activities of the animals and their emotional state; the state of their motor and vestibular sphere was improved. Thus, citicoline, when introduced for seven days after reperfusion, positively affects the recovery of nerve tissue and the functional state of cerebral neurons within the ischemic penumbra.

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Correspondence to O. P. Mankivska.

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Mankivska, O.P., Chaika, N.V. & Skibo, G.G. Effects of Citicoline on Structural/Functional Consequences of Focal Ischemia of the Rat Brain. Neurophysiology 53, 78–87 (2022).

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  • focal cerebral ischemia/reperfusion
  • cerebral infarction
  • citicoline
  • infarction volume
  • neurological deficiency scale
  • behavioral tests
  • rat