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NADPH Oxidase and Superoxide-Producing Associates in Cells of the Spinal Cord and Bone Marrow in Diabetic Rats with Spinal Cord Injury

For the first time, a method for isolation and purification of the total fraction of NOX isoforms (NOX1 + NOX2) and the total fraction of O2 producing stable associates of NOX and NADPH isoformscontaining lipoprotein (NCL associate) from the membranes of cells and intracellular formations of the spinal cord (SC) and bone marrow (BM) was developed in a rat model of fructose-induced diabetes. Characteristic changes of the optic spectra related to the level of NOX and associates were examined. Thus, an important source of O2 radicals under conditions of oxidative stress, namely, NOX and its associates, has been identified in experiments on rats. It was found that the amounts of NOX and its associates and, respectively, the activity of these biochemical systems in animals with experimental fructose-induced type II diabetes are significantly greater than those in control rats. In turn, the respective indices in diabetic rats with additional severe impairment of the spinal cord (partial transection) are considerably higher than those in diabetic rats with no spinal cord trauma. Such findings confirm the statement that oxidative stress is a significant pathogenetic factor under conditions of both diabetes and spinal cord injury. Possible applications of the obtained experimental data in the treatment of the above pathological events are discussed.

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Simonyan, K.V., Chavushyan, V.A., Lorikyan, A.G. et al. NADPH Oxidase and Superoxide-Producing Associates in Cells of the Spinal Cord and Bone Marrow in Diabetic Rats with Spinal Cord Injury. Neurophysiology 52, 423–429 (2020). https://doi.org/10.1007/s11062-021-09900-w

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Keywords

  • NOX and NADPH isoforms
  • superoxide-producing associates
  • spinal cord
  • bone marrow
  • diabetes
  • spinal cord injury
  • optic spectra