We studied changes in the expression of neurofilament protein (NFP) in the rat sensorimotor cortex after microembolization in the vessels of one hemisphere using unilateral injection of a suspension of adipocytes into the common carotid artery. The expression of NFP was estimated by the results of immunohistochemical detection of NFP using monoclonal murine antibodies against human NFP (clone 2F11, Dako, Denmark). In control rats, NFP was identified predominantly within thin terminals of nerve fibers but not in neuronal perikaryons. After microembolization of the vessels of the left cerebral hemisphere, we observed a total decrease in the intensity of expression of NFP; a mosaic pattern of the loci with practical absence and intensification of such expression could be noted. Within late time intervals after microembolization, we sporadically found labled NFP structures in the perikaryons, axon hillocks, and axons of some neurons. Changes in the expression of NFP demonstrated a widespread pattern (they were also observed in neocortical parts of the contralateral hemisphere). Course injections of the immunomodulator Imunofan resulted, in the whole, in a decrease in the intensity of pathological changes in the expression of NFP. This is indicative of a possibility for the involvement of immune processes in the pathogenesis of the observable changes. Within the recovery period after microembolization, we observed a considerable number of NFP positive bodies in the neocortex, which can be qualified as growth bulbs (cones).
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I. Zozulya, G. O. Slabkyi, and A. S. Zozulya, “Problems facing researchers, which are related to cerebrovascular diseases, in whole, and to insult, in particular,” Ukr. Med. Chasopys, 103, No. 5, 112-120 (2014).
Z. A. Suslina and M. A. Piradov, Insult: Diagnostics, Treatment, and Prophylaxis [in Russian], MEDpressinform, Moscow (2008).
V. V. Semenchenko, S. S. Stepanov, and N. N. Bogolepov, Synaptic Plasticity of the Brain (Fundamental and Applied Aspects) [in Russian], Direkt-Media, Moscow (2014).
S. Takeda, M. Inui, S. Tamaki, et al., “Electron ion correlation in liquid magnesium,” J. Phys. Soc. Jpn., 63, No. 5, 1794-1802 (1994).
Perrone Capano, R. Pernas-Alonso, and U. di Porzio, “Neurofilament homeostasis and motoneurone degeneration,” BioEssay, 23, No. 1, 24-33 (2001).
E. Schroeder, S. Vogelgesang, A. Popa-Wagner, and C. Kessler, “Neurofilament expression in the rat brain after cerebral infarction: effect of age,” J. Neurobiol. Aging, 24, No. 1, 135-145 (2003).
N. Grabovoy and L. M. Yaremenko, “The state of neocortex in the course of modeling of blood supply disturbance and after correction of concomitant changes in the immune system in rats,” Nauk. Visn. Bogomolets Nat. Med. Univ., No. 4, 28-33 (2009).
E. I. Gusev and V. I. Skvortsova, Ischemia of the Brain [in Russian], Meditsina, Moscow (2001).
L. M. Yaremenko and A. N. Grabovoy, “The state of titres of autoantibodies against tissue antigens of the brain and circulating immune complexes in the course of modeling of blood supply disturbance of the brain of different degrees of severity and its correction,” Imunol. Alergol. (Kyiv), Nos. 2/3, 55-59 (2009).
Á. Chamorro, A. Meisel, A. M. Planas, et al., “The immunology of acute stroke,” Nat. Rev. Neurol., 8, 401-410 (2012).
V. V. Lebedev and S. A. Novikov, “Hydrophilic hexapeptide Imunofan – hyperactive regulator of transport peptides of multidrug resistance,” Byull. Éksp. Biol. Med., 142, No. 12, 649-651 (2006).
V. Karaulov, “Molecular/biological substantiation of application of Imunofan in clinical practice,” Lechashchii Vrach, No. 4, 46-47 (2000).
P. D. Hurn and I. M. Macrae, “Estrogen as a neuroprotectant in stroke,” J. Cerebr. Blood Flow Metab., 20, No. 4, 631-652 (2000).
Patent No. 34604, Ukraine, MPK G09B 23/00, The Technique for Modeling of Ischemic Damage to the Brain [in Ukrainian], A. N. Grabovoy, L. M. Yaremenko, and N. G. Panishina, Publ. 11.08.08, Bull. No. 15.
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Yaremenko, L.M., Grabovoy, A.N. Changes in the Expression of Neurofilament Protein in the Rat Sensorimotor Cortex Induced by Microembolization of Blood Vessels: Effect of Immunomodulation. Neurophysiology 48, 111–116 (2016). https://doi.org/10.1007/s11062-016-9576-8
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DOI: https://doi.org/10.1007/s11062-016-9576-8