We estimated expression of the c-fos gene (a marker of increase in neuronal activity) and manifestations of the histochemical reaction to NADPH-diaphorase (a marker of NO-generating neurons) in the medial prefrontal cortex (MPFC) and forebrain limbic structures of rats in the norm, in the state of starvation, and during realization of long-lasting (60 min) periodic (several times per minute) food-procuring movements of the forelimb. The starvation state or realization of motivated stereotyped forelimb movements were related to significant bilateral increases (P < 0.05) in the levels of c-fos expression in the anterior olfactory (AOP) and cortical (ACo) nuclei and the central (Ce) and basolateral (BLA) nuclei of the amygdala, and also in the pyriform (Pyr), prelimbic (PrL), and inferior limbic (IL) cortices. The described findings demonstrate that the high Fos immunoreactivity in the MPFC and amygdalar structures is related to the motivation state in animals and reflects the active involvement of limbic cerebral structures in the formation of motor programs and also in the stabilization and realization of operant reflexes.
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References
C. Herry and N. Mons, “Resistance to extinction is associated with impaired immediate early gene induction in medial prefrontal cortex and amygdala,” Eur. J. Neurosci., 20, 781–790 (2004).
O. V. Vlasenko, V. M. Moroz, M. V. Yoltukhovskii, et al., “Activity of neurons of the cortex and subcortical cerebral structures at preparing for and realization of the movements,” Fiziol. Zh., 52, No. 2, 27 (2006).
O. V. Vlasenko, V. A. Maiskii, A. V. Maznychenko, et al., “Study of the c-fos expression and NADPH-diaphorase activity in the spinal cord and brain at the development of fatigue of the neck muscles in rats,” Fiziol. Zh., 52, No. 6, 3–14 (2006).
A. V. Maznychenko, A. I. Pilyavskii, A. I. Kostyukov, et al., “Coupling of c-fos expression in the spinal cord and amygdala induced by dorsal neck muscles fatigue,” Histochem. Cell Biol., 128, 85–90 (2007).
D. Ongь and J. L. Price, “The organization of networks within the orbital and medial prefrontal cortex of rats, monkeys, and humans,” Cerebr. Cortex, 10, 206–219 (2000).
O. V. Dovgan', V. A. Maiskii, A. I. Pilyavskii, and A. V. Maznychenko, “NADPH diaphorase-reactive neurons and their interrelation with microvessels in the basal limbic forebrain structures and hypothalamus,” Fiziol. Zh., 53, No. 5, 35–46 (2007).
S. Miyachi, X. Lu, S. Inoue, et al., “Organization of multisynaptic inputs from prefrontal cortex to primary motor cortex as revealed by retrograde transneuronal transport of rabies virus,” J. Neurosci., 25, 2547–2556 (2005).
R. E. Nordquist, C. M. Pennartz, H. B. Uylings, et al., “C-fos activation patterns in rat prefrontal cortex during acquisition of a cued classical conditioning task,” Behav. Brain Res., 146, 65–75 (2003).
S. Matsuda, H. Peng, H. Yoshimura, et al., “Persistent c-fos expression in the brains of mice with chronic social stress,” Neurosci. Res., 26, 157–170 (1996).
G. Paxinos and C. Watson, The Rat Brain in Stereotaxic Coordinates, Academic Press, San Diego (1997).
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Neirofiziologiya/Neurophysiology, Vol. 40, No. 3, pp. 256–259, May–June, 2008.
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Vlasenko, O.V., Dovgan’, O.V. Expression of c-fos as an index of functional interaction of the frontal cortex and limbic cerebral structures in the course of food-procuring stereotyped movements in rats. Neurophysiology 40, 217–219 (2008). https://doi.org/10.1007/s11062-008-9037-0
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DOI: https://doi.org/10.1007/s11062-008-9037-0