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Modulation of A-type potassium current in smooth-muscle cells of the rat Vas Deferens by Ca2+/calmodulin-dependent protein kinase II

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Abstract

Using a standard patch-clamp technique in the perforated patch configuration, we studied the effect of a highly specific membrane-permeable inhibitor of Ca2+/calmodulin-dependent protein kinase II (CaM-KII), KN-93, on fast outward A-type potassium current in isolated smooth-muscle cells (SMCs) of an epididymal region of the rat vas deferens. This inhibitor significantly changed the dynamics of the studied current; in particular, it increased the rate of inactivation and considerably slowed down the recovery after inactivation. In the presence of 5 µM KN-93, we observed a moderate (nearly by 20%) decrease in the peak amplitude of fast A-type current. Based on the data obtained, we conclude that voltage-sensitive fast A-type potassium current in SMCs of the epididymal part of the rat vas deferens can be significantly regulated by the activity of CaM-KII. Therefore, by influencing the kinetic characteristics of the above current, this enzyme can be indirectly involved in the control of electrical activity in SMCs.

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References

  1. M. I. Harhun, A. Jurkiewicz, N. H. Jurkiewicz, et al., “Voltage-gated potassium currents in rat vas deferens smooth muscle cells,” Pflügers Arch.-Eur. J. Physiol., 446, 380–386 (2003).

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  2. S. Tessier, P. Karczewski, E. G. Krause, et al., “Regulation of the transient outward K+ current by Ca2+/calmodulin-dependent protein kinase II in human atrial myocytes,” Circ. Res., 85, 810–819 (1999).

    PubMed  CAS  Google Scholar 

  3. S. D. Koh, B. A. Perrino, W. J. Hatton, et al., “Novel regulation of the A-type K+ current in murine proximal colon by calcium-calmodulin-dependent protein kinase II,” J. Physiol., 517, 75–84 (1999).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    D. O. Kryshtal’, V. V. Nesin & M. F. Shuba

Authors
  1. D. O. Kryshtal’
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  2. V. V. Nesin
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  3. M. F. Shuba
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Correspondence to D. O. Kryshtal’.

Additional information

Neirofiziologiya/Neurophysiology, Vol. 39, Nos. 4/5, pp. 419–422, July–October, 2007.

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Kryshtal’, D.O., Nesin, V.V. & Shuba, M.F. Modulation of A-type potassium current in smooth-muscle cells of the rat Vas Deferens by Ca2+/calmodulin-dependent protein kinase II. Neurophysiology 39, 369–372 (2007). https://doi.org/10.1007/s11062-007-0060-3

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  • DOI: https://doi.org/10.1007/s11062-007-0060-3

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