Skip to main content
SpringerLink
Log in

Visualizing recycling of synaptic vesicle proteins

  • Published:
Neurophysiology Aims and scope

Abstract

The use of modern techniques (in particular, novel fluorescence markers of a few molecular participants of the exo-and endocytotic processes, including pH-sensitive agents, immuno-electron and laser-scanning microscopy) allows experimenters to visualize different stages of recycling of synaptic vesicle proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. J. E. Heuser and T. S. Reese, “Evidence for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction,” J. Cell Biol., 57, No. 2, 315–344 (1973).

    Article  PubMed  CAS  Google Scholar 

  2. B. Ceccarelli, W. P. Hurlbut, and A. Mauro, “Turnover of transmitter and synaptic vesicles at the frog neuromuscular junction,” J. Cell Biol., 57, No. 2, 499–524 (1973).

    Article  PubMed  CAS  Google Scholar 

  3. K. I. Willig et al., “STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis,” Nature, 440, No. 7086, 935–939 (2006).

    Article  PubMed  CAS  Google Scholar 

  4. M. Wienisch and J. Klingauf, “Vesicular proteins exocytosed and subsequently retrieved by compensatory endocytosis are nonidentical,” Nat. Neurosci., 9, No. 8, 1019–1027 (2006).

    Article  PubMed  CAS  Google Scholar 

  5. T. Fernandez-Alfonso, R. Kwan, and T. A. Ryan, “Synaptic vesicles interchange their membrane proteins with a large surface reservoir during recycling,” Neuron, 51, No. 2, 179–186 (2006).

    Article  PubMed  CAS  Google Scholar 

  6. J. S. Dittman and J. M. Kaplan, “Factors regulating the abundance and localization of synaptobrevin in the plasma membrane,” Proc. Natl. Acad. Sci. USA, 103, No. 30, 11399–11404 (2006).

    Article  PubMed  CAS  Google Scholar 

  7. G. Miesenbock, D. A. De Angelis, and J. E. Rothman, “Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins,” Nature, 394, No. 6689, 192–195 (1998).

    Article  PubMed  CAS  Google Scholar 

  8. S. Sankaranarayanan et al., “The use of pHluorins for optical measurements of presynaptic activity,” Biophys. J., 79, No. 4, 2199–2208 (2000).

    Article  PubMed  CAS  Google Scholar 

  9. P. Taubenblatt et al., “VAMP (synaptobrevin) is present in the plasma membrane of nerve terminals,” J. Cell Sci., 112, Part 20, 3559–3567 (1999).

    PubMed  CAS  Google Scholar 

  10. E. A. Lemke and J. Klingauf, “Single synaptic vesicle tracking in individual hippocampal boutons at rest and during synaptic activity,” J. Neurosci., 25, No. 47, 11034–11044 (2005).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Max-Planck Institut Biophysikalische Chemie, Güttingen, Germany

    J. Klingauf

Authors
  1. J. Klingauf
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. Klingauf.

Additional information

Neirofiziologiya/Neurophysiology, Vol. 39, Nos. 4/5, pp. 350–351, July–October, 2007.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Klingauf, J. Visualizing recycling of synaptic vesicle proteins. Neurophysiology 39, 305–306 (2007). https://doi.org/10.1007/s11062-007-0041-6

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11062-007-0041-6

Keywords

Search

Navigation