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CHD5 gene variant predicts leptomeningeal metastasis after surgical resection of brain metastases of breast cancer

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Abstract

Purpose

Leptomeningeal metastasis (LM) is a complication of surgery for brain metastasis and is a risk factor of poor prognosis. The risk of LM is particularly high after surgery for a breast cancer metastasis to the brain. If the risk of LM after surgical resection for a brain metastasis were predictable, appropriate adjuvant therapy could be administered to individual patients to improve their prognosis. The present study aimed to reveal the genetic characteristics of brain metastases as means of predicting LM in breast cancer patients.

Methods

Ten patients with brain metastases of breast cancer presented LM after surgical resection were analyzed by whole-exome sequencing.

Results

A chromodomain-helicase-DNA-binding protein 5 (CHD5) gene alteration was detected in nine cases (90%), including a nonsynonymous variant in four cases and copy number deletion in five cases. CHD5 protein expression was lost in nine cases and had decreased in one case. The frequency of CHD5 gene alteration in brain metastases with LM was significantly higher than in primary breast cancer (2.3%) or in brain metastases of breast cancer (0%) (p < 0.0001).

Conclusions

These results suggested that the CHD5 gene alteration was associated with LM after surgical resection of breast cancer brain metastases. Searching for the gene alteration might predict the LM risk after surgical resection.

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Data Availability

The datasets analyzed during the current study are available from the corresponding author on reasonable request.

Code Availability

Not applicable.

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Acknowledgements

We are grateful to the patients for donating their tissue to research.

Funding

This study was supported by the Clinical Research Fund of the Tokyo Metropolitan Government (R030303007).

Author information

Authors and Affiliations

  1. Department of Neurosurgery, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo, 113-8677, Japan

    Ryohei Otani, Ryoji Yamada, Hirohisa Yajima, Shinji Kawamura & Sakura Shimizu

  2. Clinical Research Support Center, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo, 113-8677, Japan

    Daichi Sadato

  3. Department of Neurosurgery, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan

    Shota Tanaka, Shunsaku Takayanagi & Hirokazu Takami

  4. Department of Clinical Genetics, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo, 113-0021, Japan

    Tatsuro Yamaguchi

Authors
  1. Ryohei Otani
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Contributions

Designed and performed the study: R.O. Sample correction: R.O., R.Y., H.Y. Analysis and interpretation of whole exome sequence data: D.S., R.O. Manuscript: R.O., D.S. Review and discussion of the manuscript: all the authors.

Corresponding author

Correspondence to Ryohei Otani.

Ethics declarations

Ethics approval

The present study was approved by the ethics committee at Tokyo Metropolitan Komagome Hospital (No. 2631).

Consent to participate

Written informed consent was obtained from all individual participants included in the study.

Consent for publication

Patients signed informed consent regarding publishing their data.

Conflicts of Interest/Competing interests

The authors declare that they have no competing interests.

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Electronic supplementary material

Below is the link to the electronic supplementary material.

11060_2023_4381_MOESM1_ESM.docx

Supplementary Table S1. All pathogenic variants detected in ten cases of breast cancer-related brain metastases with leptomeningeal metastasis after surgical resection. VAF, Variant allele frequency.

11060_2023_4381_MOESM2_ESM.docx

Supplementary Table S2. Frequency of CHD5 gene variants in brain metastases and primary lesions of various cancer types other than breast cancer in the TCGA data.

11060_2023_4381_MOESM3_ESM.tif

Supplementary Figure S1. Immunohistochemical staining of the CHD5 protein in all the samples of breast cancer-related brain metastasis with leptomeningeal metastasis in the present study. The positive control is also shown.

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Otani, R., Sadato, D., Yamada, R. et al. CHD5 gene variant predicts leptomeningeal metastasis after surgical resection of brain metastases of breast cancer. J Neurooncol 163, 657–662 (2023). https://doi.org/10.1007/s11060-023-04381-9

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  • DOI: https://doi.org/10.1007/s11060-023-04381-9

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