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LncRNA GAS5 regulates the proliferation, migration, invasion and apoptosis of brain glioma cells through targeting GSTM3 expression. The effect of LncRNA GAS5 on glioma cells

  • Clinical Study
  • Published:
Journal of Neuro-Oncology Aims and scope Submit manuscript

Abstract

Introduction

To investigate the effects of lncRNA GAS5 on the proliferation, migration, invasion and apoptosis of brain glioma cells.

Methods

The expression levels of lncRNA GAS5 and GSTM3 in normal glial cells (HEB) and glioma cells (U251 and U87) were detected by RT-qPCR and western blot, respectively. Glioma cells were transfected with ctrl vector, pcDNA-GAS5, siRNA ctrl (siNC) or GSTM3 siRNA and the effects of lncRNA GAS5 and GSTM3 on the proliferation, migration, invasion and apoptosis of glioma cells were detected by CCK-8 assay, transwell assay and Caspase 3/7 activity assay, respectively.

Results

The expression of lncRNA GAS5 was significantly decreased in glioma cell lines U251 and U87 compared with normal glial cells HEB (p < 0.01). In addition, overexpression of lncRNA GAS5 inhibited the proliferation, migration and invasion of U251 and U87 cells, and promoted cell apoptosis as demonstrated by the increased activity of Caspase 3/7. Furthermore, GSTM3 was predicted as a target gene of lncRNA GAS5 by bioinformatics analysis and its expression was increased in glioma cells compared with the normal cells as indicated by western blotting and RT-qPCR experimental results. Silencing of GSTM3 with GSTM3 siRNA decreased the proliferation, migration and invasion but increased the apoptosis of glioma cell lines U251 and U87, which was similar to that the effect lncRNA GAS5 over-expression.

Conclusion

lncRNA GAS5 can effectively inhibit the proliferation, migration and invasion of glioma cells and promote cell apoptosis through targeting GSTM3 expression.

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Data availability

All data generated or analysed during this study are included in this published article.

Abbreviations

siNC:

siRNA ctrl

Lnc:

Long non-coding

GAS5:

Growth-arrest specific transcript 5

GSTM3:

Glutathione-S-transferase M3

BCA:

Bicinchoninic acid

NC:

Nitrocellulose

CCRCC:

Clear cell renal cell carcinoma

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Acknowledgements

This study was supported by the Innovation of Science and Technology Committee of Shenzhen City (Grant Number: JCYJ20150402155418386) and the Projects of Public Welfare Research and Capacity Development of Guangdong Province, China (Grant Number: 2014A020212171).

Author information

Authors and Affiliations

  1. Department of Neurosurgery, People’s Hospital of Shiyan, Shenzhen, China

    Guoxiong Li

  2. Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory On Brain Function Repair and Regeneration, Guangzhou, China

    Guoxiong Li, Yingqian Cai, Min Huang & Jiansheng Chen

  3. Department of Nutrition, Affiliated Baoan Hospital of Shenzhen, Southern Medical University, Shenzhen, 518101, China

    Chuanmei Wang

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  1. Guoxiong Li
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Correspondence to Chuanmei Wang or Min Huang.

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Li, G., Cai, Y., Wang, C. et al. LncRNA GAS5 regulates the proliferation, migration, invasion and apoptosis of brain glioma cells through targeting GSTM3 expression. The effect of LncRNA GAS5 on glioma cells. J Neurooncol 143, 525–536 (2019). https://doi.org/10.1007/s11060-019-03185-0

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