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Monitoring of intracerebellarly-administered natural killer cells with fluorine-19 MRI

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A Correction to this article was published on 09 April 2019

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Abstract

Purpose

Medulloblastoma (MB) is the most common malignant brain tumor in children. Recent studies have shown the ability of natural killer (NK) cells to lyse MB cell lines in vitro, but in vivo successes remain elusive and the efficacy and fate of NK cells in vivo remain unknown.

Methods

To address these questions, we injected MB cells into the cerebellum of immunodeficient mice and examined tumor growth at various days after tumor establishment via bioluminescence imaging. NK cells were labeled with a fluorine-19 (19F) MRI probe and subsequently injected either intratumorally or contralaterally to the tumor in the cerebellum and effect on tumor growth was monitored.

Results

The 19F probe efficiently labeled the NK cells and exhibited little cytotoxicity. Fluorine-19 MRI confirmed the successful and accurate delivery of the labeled NK cells to the cerebellum of the mice. Administration of 19F–labeled NK cells suppressed MB growth, with the same efficacy as unlabeled cells. Immunohistochemistry confirmed the presence of NK cells within the tumor, which was associated with induction of apoptosis in tumor cells. NK cell migration to the tumor from a distal location as well as activation of apoptosis was also demonstrated by immunohstochemistry.

Conclusions

Our results show that NK cells present a novel opportunity for new strategies in MB treatment. Further, 19F-labeled NK cells can suppress MB growth while enabling 19F MRI to provide imaging feedback that can facilitate study and optimization of therapeutic paradigms.

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Change history

  • 09 April 2019

    There was a typo in author Andrew Wahba’s name in the initial online publication. The original article has been corrected.

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Acknowledgements

This work was supported by funding support from Addis Faith Foundation, Noah’s Light Foundation, American Cancer Society Award Number 118165-RSG-09-273-01-DDC and the Rally Foundation for Childhood Cancers to VG.

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Authors and Affiliations

  1. Division of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA

    Bridget A. Kennis, William B. Brugmann, Alvaro Laureano, Rong-Hua Tao, Srinivas S. Somanchi, Javiera B. Bravo-Alegria, Shinji Maegawa, Andrew Wahba, Simin Kiany, Nancy Gordon, Soumen Khatua, Wafik Zaky, David Sandberg, Laurence Cooper, Dean A. Lee & Vidya Gopalakrishnan

  2. Department of Imaging Physics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA

    Keith A. Michel, Samuel A. Einstein & James A. Bankson

  3. Diagnostic Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA

    Dawid Schellingerhout

  4. Division of Transplantation and Cellular Therapy, Hospital das Clinicas de Porto Alegre, Federal University of Rio Grande do Sul, Porto Alegre, Brazil

    Alvaro Laureano & Lucia Silla

  5. Ziopharm Oncology, Boston, MA, USA

    Laurence Cooper

  6. Department of Pediatric Neurosurgery, University of Texas Health Science Center, Houston, TX, USA

    David Sandberg

  7. Department of Pediatrics, Ohio State University, 700 Children’s Dr, Columbus, OH, 43205, USA

    Dean A. Lee

  8. Division of Pediatrics, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Unit 0853, Houston, TX, 77030, USA

    Vidya Gopalakrishnan

  9. Department of Imaging Physics, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Unit 1902, Houston, TX, 77030, USA

    James A. Bankson

Authors
  1. Bridget A. Kennis
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  2. Keith A. Michel
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  19. Dean A. Lee
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Corresponding authors

Correspondence to Dean A. Lee, James A. Bankson or Vidya Gopalakrishnan.

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Research involving human and animal participants

All animal experiments were approved by Institutional Animal Care and Use Committee (IACUC).

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The original version of this article has been revised: Author Andrew Wahba's name has been corrected.

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Kennis, B.A., Michel, K.A., Brugmann, W.B. et al. Monitoring of intracerebellarly-administered natural killer cells with fluorine-19 MRI. J Neurooncol 142, 395–407 (2019). https://doi.org/10.1007/s11060-019-03091-5

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