Abstract
Long non-coding RNAs (lncRNAs) are important modulators of various cellular and molecular events, including cancer-associated pathways. The Anti-differentiation ncRNA (ANCR) is a key regulator of keratinocyte differentiation, where its expression is necessary to maintain epidermal progenitor’s cells. Herein, we investigated the expression pattern of ANCR in the course of neural differentiation. Moreover, we used published RNAseq data and clinical samples to evaluate the alteration of ANCR expression in different cell types and brain tumors. Furthermore, we manipulated ANCR expression in glioma cell lines to clarify a potential functional role for ANCR in tumorigenesis. Our qRT-PCR results revealed a significant upregulation of ANCR in more malignant and less differentiated types of brain tumors (P = 0.03). This data was in accordance with down regulation of ANCR during neural differentiation. ANCR suppression caused an elevation in apoptosis rate, as well as a G1 cell cycle arrest in glioblastoma cell line. Altogether, our data demonstrated that ANCR may play a role in glioma genesis and that it could be considered as a potential diagnostic and therapeutic target to combat brain cancers.
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Acknowledgements
We are very thankful to Dr. Mohammad Malakootian, Dr. Zahra Bahadori, Ms Parisa Naeli, Ms Mozhgan Saadat, Ms. Hayat and Ms. Mohseni for their excellent advices and technical assistances. This work was supported by a research grant from Iran National Science Foundation.
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11060_2018_2809_MOESM1_ESM.jpeg
Supplementary Figure 1: The expression pattern of ANCR in TCGA Glioblastoma multiforme (GBM) samples. To evaluate the expression of ANCR lncRNA in larger sample size we have used TANRIC to calculate the expression level in TCGA RNA sequencing data. TANRIC analyzed 154 samples for GBM in 5 different subtypes (reported P value: 0.000031827) (JPEG 58 KB)
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Malakootian, M., Mirzadeh Azad, F., Fouani, Y. et al. Anti-differentiation non-coding RNA, ANCR, is differentially expressed in different types of brain tumors. J Neurooncol 138, 261–270 (2018). https://doi.org/10.1007/s11060-018-2809-5
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DOI: https://doi.org/10.1007/s11060-018-2809-5