Journal of Neuro-Oncology

, Volume 133, Issue 2, pp 257–264 | Cite as

Precision knockdown of EGFR gene expression using radio frequency electromagnetic energy

  • Ilya V. Ulasov
  • Haidn Foster
  • Mike Butters
  • Jae-Geun Yoon
  • Tomoko Ozawa
  • Theodore Nicolaides
  • Xavier Figueroa
  • Parvinder Hothi
  • Michael Prados
  • John Butters
  • Charles Cobbs
Laboratory Investigation

Abstract

Electromagnetic fields (EMF) in the radio frequency energy (RFE) range can affect cells at the molecular level. Here we report a technology that can record the specific RFE signal of a given molecule, in this case the siRNA of epidermal growth factor receptor (EGFR). We demonstrate that cells exposed to this EGFR siRNA RFE signal have a 30–70% reduction of EGFR mRNA expression and ~60% reduction in EGFR protein expression vs. control treated cells. Specificity for EGFR siRNA effect was confirmed via RNA microarray and antibody dot blot array. The EGFR siRNA RFE decreased cell viability, as measured by Calcein-AM measures, LDH release and Caspase 3 cleavage, and increased orthotopic xenograft survival. The outcomes of this study demonstrate that an RFE signal can induce a specific siRNA-like effect on cells. This technology opens vast possibilities of targeting a broader range of molecules with applications in medicine, agriculture and other areas.

Keywords

Electromagnetic energy Radio frequency EGFR 

Supplementary material

11060_2017_2440_MOESM1_ESM.docx (564 kb)
Supplementary material 1 (DOCX 564 KB)

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Ilya V. Ulasov
    • 1
  • Haidn Foster
    • 1
  • Mike Butters
    • 2
  • Jae-Geun Yoon
    • 1
  • Tomoko Ozawa
    • 3
  • Theodore Nicolaides
    • 3
  • Xavier Figueroa
    • 2
  • Parvinder Hothi
    • 1
  • Michael Prados
    • 3
  • John Butters
    • 2
  • Charles Cobbs
    • 1
  1. 1.Ben & Catherine Ivy Center for Advanced Brain Tumor TreatmentSwedish Neuroscience InstituteSeattleUSA
  2. 2.Nativis Inc.SeattleUSA
  3. 3.Department of Neurosurgery, Brain Tumor Research CenterUniversity of California San FranciscoSan FranciscoUSA

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