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Effect of 10 different polymorphisms on preoperative volumetric characteristics of glioblastoma multiforme

Journal of Neuro-Oncology volume 126, pages 585–592 (2016)Cite this article

Abstract

There is a distinct diversity between the appearance of every glioblastoma multiforme (GBM) on pretreatment magnetic resonance imaging (MRI) with a potential impact on clinical outcome and survival of the patients. The object of this study was to determine the impact of 10 different single nucleotide polymorphisms (SNPs) on various volumetric parameters in patients harboring a GBM. We prospectively analyzed 20 steroid-naïve adult patients who had been treated for newly diagnosed GBM. The volumetry was performed using MRI with the help of a semiautomated quantitative software measuring contrast enhancing tumor volume including necrosis, central necrosis alone and peritumoral edema (PTE). We calculated ratios between the tumor volume and edema (ETR), respectively necrosis (NTR). SNP analysis was done using genomic DNA extracted from peripheral blood genotyped via PCR and sequencing. There was a strong correlation between tumor volume and PTE (p < 0.001), necrosis (p < 0.001) and NTR (p = 0.003). Age and sex had no influence on volumetric data. The Aquaporin 4-31G > A SNP had a significant influence on the ETR (p = 0.042) by decreasing the measured edema compared with the tumor volume. The Interleukin 8-251A > T SNP was significantly correlated with an increased tumor (p = 0.048), PTE (p = 0.033) and necrosis volume (p = 0.028). We found two SNPs with a distinct impact on pretreatment tumor characteristics, presenting a potential explanation for the individual diversity of GBM appearance on MRI and influence on survival.

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Acknowledgments

We thank Dr. Maryam Sherman for her kind help in editing our draft.

Funding

No financial support was provided for this study.

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Affiliations

  1. Department of Neurosurgery, University Hospital Rostock, Schillingallee 35, 18057, Rostock, Germany

    Christian Henker, Thomas Kriesen & Jürgen Piek

  2. Institute of Experimental Gene Therapy and Cancer Research, Rostock University Medical Center, Schillingallee 69, 18057, Rostock, Germany

    Katharina Fürst, Deborah Goody & Brigitte M. Pützer

  3. Institute for Biostatistics and Informatics in Medicine, Rostock University Medical Center, Ernst-Heydemann-Str. 8, 18057, Rostock, Germany

    Änne Glass

Authors
  1. Christian Henker
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  2. Thomas Kriesen
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  3. Katharina Fürst
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  4. Deborah Goody
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  5. Änne Glass
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  6. Brigitte M. Pützer
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Correspondence to Christian Henker.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Henker, C., Kriesen, T., Fürst, K. et al. Effect of 10 different polymorphisms on preoperative volumetric characteristics of glioblastoma multiforme. J Neurooncol 126, 585–592 (2016). https://doi.org/10.1007/s11060-015-2005-9

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  • DOI: https://doi.org/10.1007/s11060-015-2005-9

Keywords

  • Glioblastoma
  • Volumetrics
  • Edema
  • Necrosis
  • Polymorphism