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Galectin-3 in preneoplastic lesions of glioma

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Abstract

Gliomas are the most common primary brain tumors in adults and have a poor prognosis. Galectin-3 is a β-galactosidase-binding lectin which is important in pre-mRNA splicing, regulation of cell proliferation, cell adhesion and apoptosis. Although galectin-3 has been shown as a glioma related marker and expression of galectin-3 has been reported to correlate with WHO grade in human gliomas, expression of galectin-3 in early neoplastic lesions such as early neoplastic proliferation (ENP) and microtumor is still far from fully understood. In the present study, expression of galectin-3 in ethylnitrosourea-induced rat gliomas including preneoplastic and neoplastic lesions was examined by immunohistochemistry for galectin-3, Iba-1 (a specific microglial cell marker), GFAP (a specific astrocyte cell marker), and conventional hematoxylin and eosin staining (for morphological observation). The results showed that exact location of ENP was detected clearly by galectin-3 immunohistochemistry whereas normal brain tissues were negative. In ENP and microtumor, galectin-3 was expressed in neoplastic astrocytic cells but rarely in microglia. In malignant glioma, however, galectin-3 was expressed in both neoplastic astrocytic cells and microglia. This suggests that galectin-3 is activated in microglia and macrophages according to the progression of glioma. Galectin-3 was not expressed in oligodendrocytic cells. Our results indicate that galectin-3 is a good specific marker indicating the early stage of glioma tumorigenesis.

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Acknowledgments

The authors are indebted to Ms. Kyoko Takahashi for her excellent technical assistance. This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

Ethical standards

We fully complied with the ‘Guidelines Concerning Experimental Animals’ issued by the Japanese Association for Laboratory Animal Science and exercised due consideration so as not to cause any ethical problems.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Tumor Pathology, Gifu University Graduate School of Medicine, Yanagido 1-1, Gifu City, Gifu, 501-1194, Japan

    Nguyen Huy Binh, Kunio Satoh, Kazuhiro Kobayashi, Manabu Takamatsu, Yuichiro Hatano, Akihiro Hirata, Hiroyuki Tomita, Toshiya Kuno & Akira Hara

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  1. Nguyen Huy Binh
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  2. Kunio Satoh
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  3. Kazuhiro Kobayashi
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  4. Manabu Takamatsu
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  6. Akihiro Hirata
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  7. Hiroyuki Tomita
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  9. Akira Hara
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Correspondence to Akira Hara.

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Binh, N.H., Satoh, K., Kobayashi, K. et al. Galectin-3 in preneoplastic lesions of glioma. J Neurooncol 111, 123–132 (2013). https://doi.org/10.1007/s11060-012-1005-2

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  • DOI: https://doi.org/10.1007/s11060-012-1005-2

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