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Journal of Neuro-Oncology

, Volume 110, Issue 1, pp 69–77 | Cite as

Expression of VAV1 in the tumour microenvironment of glioblastoma multiforme

  • Juan Luis GarciaEmail author
  • Jose Couceiro
  • Juan Antonio Gomez-Moreta
  • J. M. Gonzalez Valero
  • Angel Santos Briz
  • Vincent Sauzeau
  • Eva Lumbreras
  • Manuel Delgado
  • Cristina Robledo
  • Monica Lara Almunia
  • Xose R. Bustelo
  • Jesus M. Hernandez
Clinical Study - Patient Study

Abstract

Even though much progress has been made towards understanding the molecular nature of glioma, the survival rates of patients affected by this tumour have not changed significantly over recent years. Better knowledge of this malignancy is still needed in order to predict its outcome and improve patient treatment. VAV1 is an GDP/GTP exchange factor for Rho/Rac proteins with oncogenic potential that is involved in the regulation of cytoskeletal dynamics and cell migration. Here we report its overexpression in 59 patients diagnosed with high-grade glioma, and the associated upregulation of a number of genes coding for proteins also involved in cell invasion- and migration-related processes. Unexpectedly, immunohistochemical experiments revealed that VAV1 is not expressed in glioma cells. Instead, VAV1 is found in non-tumoural astrocyte-like cells that are located either peritumouraly or perivascularly. We propose that the expression of VAV1 is linked to synergistic signalling cross-talk between cancer and infiltrating cells. Interestingly, we show that the pattern of expression of VAV1 could have a role in the neoplastic process in glioblastoma tumours.

Keywords

Glioma Expression profile VAV1 

Notes

Acknowledgments

We thank T. Prieto, I. Rodríguez, S. González and M.A. Hernández of the Centro de Investigación del Cáncer, Salamanca, and the IECSCYL-Hospital Universitario Research Unit for their excellent technical assistance. We also thank Dr. E. Fermiñán (Genomics and Proteomics Unit) for microarray analyses.

Supplementary material

11060_2012_936_MOESM1_ESM.xls (52 kb)
Supplementary material 1 (XLS 52 kb)
11060_2012_936_MOESM2_ESM.docx (1.2 mb)
Supplementary material 2 (DOCX 1189 kb)

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Copyright information

© Springer Science+Business Media, LLC. 2012

Authors and Affiliations

  • Juan Luis Garcia
    • 1
    Email author
  • Jose Couceiro
    • 5
  • Juan Antonio Gomez-Moreta
    • 3
  • J. M. Gonzalez Valero
    • 2
  • Angel Santos Briz
    • 4
  • Vincent Sauzeau
    • 5
  • Eva Lumbreras
    • 5
  • Manuel Delgado
    • 5
  • Cristina Robledo
    • 5
  • Monica Lara Almunia
    • 3
  • Xose R. Bustelo
    • 5
  • Jesus M. Hernandez
    • 6
  1. 1.Research UnitIECSCYL-Hospital Universitario de Salamanca. IBSAL, IBMCC (USALCSIC)SalamancaSpain
  2. 2.Research UnitIECSCYL-Hospital Universitario de SalamancaSalamancaSpain
  3. 3.Neurosurgery ServiceHospital Universitario de SalamancaSalamancaSpain
  4. 4.Department of PathologyHospital Universitario de SalamancaSalamancaSpain
  5. 5.Centro de Investigación del Cáncer and Instituto de Biología Molecular y Celular del CáncerUniversidad de Salamanca-CSICSalamancaSpain
  6. 6.Centro de Investigación del Cáncer and Instituto de Biología Molecular y Celular del Cáncer IBSAL, IBMCC (USALCSIC) Department of HaematologyHospital Universitario de SalamancaSalamancaSpain

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