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The p53/p21 DNA damage-signaling pathway is defective in most meningioma cells

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Abstract

Although meningiomas represent the most common class of tumors of the central nervous system, the molecular events underlying their genesis and development are still not well defined. In the present study we have used the immuno-blotting technique to study the expression level of the tumor suppressor proteins p53, p21 and PTEN in primary meningioma cells. We have also studied the induction of p21 and p53 in response to both UV light and γ-rays. We present evidence that the p53/p21-dependent γ-ray signaling pathway is defective in 5 out of 8 (62%) of these cells. Furthermore, we have shown that the tumor suppressor p21, p53 and PTEN proteins are differently expressed in these cells, with up to 40-folds difference between the lowest and the highest levels of each protein. These results suggest that the tumor suppressors p53/p21 signaling pathway and PTEN play important roles in the development of benign meningiomas.

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Acknowledgements

We are very thankful to the Research Center administration and the Office of Research Affairs (ORA) at the KFSH&RC for their continuous support. This work was performed under the RAC proposal # 2050 015.

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Author notes

  1. Boleslaw Lach

    Present address: McMaster University, Department of Molecular and Laboratory Medicine, Hamilton Health Sciences, Hamilton General Hospital, 237 Barton Street East, Hamilton, L8L-2X2, Canada

Authors and Affiliations

  1. Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Center, MBC# 03-66, 3354, Riyadh, 11211, KSA

    Huda H. Al-Khalaf & Abdelilah Aboussekhra

  2. Department of Pathology, King Faisal Specialist Hospital and Research Center, MBC# 10, Riyadh, 11451, KSA

    Boleslaw Lach

  3. Department of Radiation Oncology, King Faisal Specialist Hospital and Research Center, MBC# 34, Riyadh, 11451, KSA

    Ayman Allam

  4. Department of Neurosciences, King Faisal Specialist Hospital and Research Center, MBC# 76, Riyadh, 11451, KSA

    Ahmed AlKhani

  5. Department of Biochemistry, King Saud University, College of Science, 2455, Riyadh, 11451, KSA

    Salman A. Alrokayan

Authors
  1. Huda H. Al-Khalaf
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  2. Boleslaw Lach
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  3. Ayman Allam
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  4. Ahmed AlKhani
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  6. Abdelilah Aboussekhra
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Corresponding author

Correspondence to Abdelilah Aboussekhra.

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Al-Khalaf, H.H., Lach, B., Allam, A. et al. The p53/p21 DNA damage-signaling pathway is defective in most meningioma cells . J Neurooncol 83, 9–15 (2007). https://doi.org/10.1007/s11060-006-9301-3

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  • DOI: https://doi.org/10.1007/s11060-006-9301-3

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