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Cell type- and region- dependent coxsackie adenovirus receptor expression in the central nervous system

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Model systems have shown that adenoviral vector mediated transient gene expression can potentially be applied for the treatment of brain tumours, neurodegenerative diseases and brain injuries. Most studies utilized adenovirus serotype 5 (Ad5) based vectors, which as adhesion molecules require the coxsackie adenovirus receptor (CAR) as a critical determinant for cellular infection. In this report, we have systematically characterized CAR expression in the adult human central nervous system (CNS) by using immunohistochemistry. A total of 85 specimens from various CNS regions were investigated for CAR expression in a cell type-dependent context. The most marked staining positivity was found in the choroid plexus and the pituitary gland. The neocortex had scattered positive neurons, while the white matter was mainly negative.

We need to consider the possible adverse effects and the possible damage caused by adenoviral gene therapy if the virus–vector also binds to normal brain cells.

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Acknowledgements

The authors thank Onyx Pharmaceuticals Inc, Richmond, CA, USA for the generous gift of the antibody and Katherine Rauen, Cancer Research Institute, University of California, San Francisco, CA, USA for mediating information. This work was supported by the Märit and Hans Rausing Charitable Foundation, the Swedish Cancer Society and Gunnar Nilsson's Cancer Foundation.

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Authors and Affiliations

  1. Department of Clinical Science, Lund, Division V, Pathology,, University Hospital, SE-221 85, Lund, Sweden

    Annette Persson & Elisabet Englund

  2. Section of Immunology,, Lund University, BMCI13, SE-221 84, Lund, Sweden

    Xiaolong Fan & Bengt Widegren

  3. Department of Cell and Organism Biology,, Lund University, Sölvegatan 35, SE-223 62, Lund, Sweden

    Bengt Widegren

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  1. Annette Persson
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  2. Xiaolong Fan
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Correspondence to Annette Persson.

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Persson, A., Fan, X., Widegren, B. et al. Cell type- and region- dependent coxsackie adenovirus receptor expression in the central nervous system. J Neurooncol 78, 1–6 (2006). https://doi.org/10.1007/s11060-005-9055-3

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  • DOI: https://doi.org/10.1007/s11060-005-9055-3

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