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Immunocytochemical detection of 14-3-3 in primary nervous system tumors

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Summary

14-3-3 proteins have attracted much recent interest in the etiopathogenesis of human cancers owing to their involvement in the prevention of apoptosis. However, the expression of 14-3-3 in primary nervous system tumors has not been previously characterized. In this paper, Immunohistochemistry using a specific anti-14-3-3 antibody was performed on formalin-fixed, paraffin embedded archival tissue from 124 primary human nervous system tumors and 10 normal brain tissues. In the normal control brains, 14-3-3 immunoreactivity was localized mainly in the neuronal somata and processes, and some glial cells showed only weak immunoreactivity. However, 14-3-3 immunoreactivity was seen in the majority of astrocytomas [grade I (9/11), II (16/21), III (13/17), IV (17/21)]. There was no difference between the positive expression rates of 14-3-3 in different grades of astrocytomas (P = 0.968). But the intensity and degree of 14-3-3 immunoreactivity in diffuse astrocytomas, anaplastic astrocytoma, and glioblastoma multiformes showed trends with tumor grade, with glioblastomas having the highest positivity (P = 0.048). The 14-3-3 immunoreactivity was also seen in the majority of other gliomas [oligodendroglioma (2/3), anaplastic oligodendroglioma (4/4), ependymoma (1/2), anaplastic ependymoma (2/2), choroid plexus papilloma (3/3), pineocytoma (2/2), medulloblastoma (5/8)]. All meningiomas [syncytical (3), fibrous/fibroblastic (4), angiomatous (4), transitional/mixed (3)] were intensely and diffusely positive. All schwannomas (4), neurofibromas (2), pituitary adenomas (6) and craniopharyngiomas(4) also showed intense positive staining. These results showed that 14-3-3 is expressed in the majority of the primary human nervous system tumors. The up-regulated expression of 14-3-3 may be a common mechanism for evading apoptosis in most primary human nervous system tumors, and targeting 14-3-3 may be a novel promising strategy for the treatment of these tumors, especially for malignant tumors.

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Acknowledgements

We thank Dr. Wei Yan and Dr. Fucheng Ma (Department of Pathology, Xijing Hospital, Fourth Military Medical University) for confirming the histopathological diagnosis of all the samples used in this research and helping to perform microscopic analysis for 14-3-3 immunoreactivity. We thank Dr. Fubo Xue’s help in statistical analysis. And we also thank Ms. Lei Song and Ms. Xiling Wang for material preparing for this research.

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Authors and Affiliations

  1. Institute of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Shaanxi Province, Xi’an, People’s Republic of China

    Wei-Dong Cao, Xiang Zhang, Jian-Ning Zhang, Hai-Ning Zhen, Guang Cheng, Bian Li & Dakuan Gao

  2. Institute of Neuromedicine, Southern Medical University, 510282, Guangzhou, People’s Republic of China

    Zhi-Jun Yang

  3. Institute of Neurosurgery, Xijing Hospital, Fourth Military Medical University, 17 Changle West Road, 710032, Shaanxi Province, Xi’an, People’s Republic of China

    Xiang Zhang

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  1. Wei-Dong Cao
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Correspondence to Xiang Zhang.

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Cao, WD., Zhang, X., Zhang, JN. et al. Immunocytochemical detection of 14-3-3 in primary nervous system tumors. J Neurooncol 77, 125–130 (2006). https://doi.org/10.1007/s11060-005-9027-7

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  • DOI: https://doi.org/10.1007/s11060-005-9027-7

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