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Meningiomas expressing and responding to cholecystokinin (CCK)

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Abstract

The effect of cholecystokinin (CCK) on cultured human meningioma derived cells was investigated. Exposure of meningioma cells for 6–12 days to CCK-8s (2–200 nM) resulted in a dose dependent stimulation of cell growth to a maximum of 1.1-fold over basal controls. A time course study showed stimulation of cell growth at day 3 followed by increase throughout day 6. The stimulatory effect of CCK on meningioma cell growth was completely abolished by a CCK-B specific receptor antagonist, L-365,260. Reverse-transcription of meningioma-derived RNA into cDNA followed by amplification by the polymerase chain reaction using specific primers for CCK peptide and its CCK-A and/B receptor revealed 100% presence of CCK peptide and CCK-B receptors mRNA whereas CCK-A receptor was expressed in 66% of the meningiomas. These results provide evidence that human meningioma cells possess CCK peptide and its receptors the activation of which leads to increase of cell growth possibly via an autocrine/paracrine mechanism.

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References

  1. T Russel T. Moss (1986) ArticleTitleMetastasizing meningioma Neurosurgery 19 1028–1030 Occurrence Handle3808235

    PubMed  Google Scholar 

  2. JW Koper EC Zwarthoff A Hagemeijer R Braakman CJ Avezaat M Bergstrom SW. Lamberts (1991) ArticleTitleInhibition of the growth of cultured human meningioma cells by recombinant interferon-alpha Eur J Cancer 4 416–419

    Google Scholar 

  3. M Kurihara Y Tokunaga K Tsutsumi T Kawaguchi K Shigematsu M Niwa K. Mori (1989) ArticleTitleCharacterization of insulin-like growth factor I and epidermal growth factor receptors in meningioma J Neursurg 4 538–544

    Google Scholar 

  4. T Nitta K Sato K. Okumura (1991) ArticleTitleTransforming growth factor (TGF)-beta like activity of intracranial meningioma and its effect on cell growth J Neurosurg Sci 1 19–23

    Google Scholar 

  5. AS Weisman JG Villemure PA. Kelly (1986) ArticleTitleRegulation of DNA synthesis and growth of cells derived from primary human meningiomas Cancer Res 5 2545–50

    Google Scholar 

  6. M Westphal HD. Herrmann (1986) ArticleTitleEpidermal growth factor–receptors on cultured human meningioma cells Acta Neurochir 1-2 62–66 Occurrence Handle10.1007/BF01420510

    Article  Google Scholar 

  7. PL Kaplan M Anderson B. Ozanne (1982) ArticleTitleTransforming growth factor(s) production enables cells to grow in the absence of serum: an autocrine system Proc Natl Acad Sci USA 2 485–489

    Google Scholar 

  8. MB Sporn AB. Roberts (1985) ArticleTitleAutocrine growth factors and cancer Nature 6005 745–747 Occurrence Handle10.1038/313745a0

    Article  Google Scholar 

  9. AS Goustin EB Leof GD Shipley HL. Moses (1986) ArticleTitleGrowth factors and cancer Cancer Res 3 1015–1029

    Google Scholar 

  10. R Kaufmann H Schafberg M Zieger P Henklein G. Nowak (1998) ArticleTitleProtein kinase C is involved in cholecystokinin octapeptide-induced proliferation action in rat glioma C6 cells Neuropeptides 2 185–189 Occurrence Handle10.1016/S0143-4179(98)90036-1

    Article  Google Scholar 

  11. JF Rehfeld WW. Solinge Particlevan (1994) ArticleTitleThe tumor biology of gastrin and cholecystokinin Adv Cancer Res 63 295–347 Occurrence Handle1:CAS:528:DyaK2MXitVagsA%3D%3D Occurrence Handle8036989

    CAS  PubMed  Google Scholar 

  12. F Lefranc C Chaboteaux N Belot J Brotchi I Salmon R. Kiss (2003) ArticleTitleDetermination of RNA expression for cholecystokinin/gastrin receptors (CCKA, CCKB and CCKC) in human tumors of the central and peripheral nervous system Int J Oncol 1 213–219

    Google Scholar 

  13. JC Reubi B Waser JC Schaer U Laederach J Erion A Srinivasan MA Schmidt JE. Bugaj (1998) ArticleTitleUnsulfated DTPA- and DOTA-CCK analogs as specific high-affinity ligands for CCK-B receptor-expressing human and rat tissues in vitro and in vivo Eur J Nucl Med 5 481–490 Occurrence Handle10.1007/s002590050247

    Article  Google Scholar 

  14. MC Beinfeld W. Wang (2002) ArticleTitleCCK processing by pituitary GH3 cells, human teratocarcinoma cells NT2 and hNT differentiated human neuronal cells evidence for a differentiation-induced change in enzyme expression and pro CCK processing Life Sci 11 1251–1258 Occurrence Handle10.1016/S0024-3205(01)01507-7

    Article  Google Scholar 

  15. TH Moran PH Robinson MS Goldrich PR. McHugh (1986) ArticleTitleTwo brain cholecystokinin receptors: implications for behavioral actions Brain Res 1 175–179 Occurrence Handle10.1016/0006-8993(86)91413-7

    Article  Google Scholar 

  16. RT Jensen JM Qian JT Lin SA Mantey JR Pisegna SA. Wank (1994) ArticleTitleDistinguishing multiple CCK receptor subtypes Studies with guinea pig chief cells and transfected human CCK receptors. Ann NY Acad Sci 713 88–106 Occurrence Handle1:CAS:528:DyaK2cXlt1GhtbY%3D

    CAS  Google Scholar 

  17. F Noble SA Wank JN Crawley J Bradwejn KB Seroogy M Hamon BP. Roques (1999) ArticleTitleInternational Union of Pharmacology. XXI Structure, distribution, and functions of cholecystokinin receptors. Pharmacol Rev 4 745–781

    Google Scholar 

  18. A Shulkes GS. Baldwin (1997) ArticleTitleBiology of gut cholecystokinin and gastrin receptors Clin Exp Pharmacol Physiol 3-4 209–216

    Google Scholar 

  19. CM Townsend SuffixJr P Singh JC. Thompson (1986) ArticleTitleGastrointestinal hormones and gastrointestinal and pancreatic carcinomas Gastroenterology 4 1002–1006

    Google Scholar 

  20. EF Adams T Lei M Buchfelder B Petersen R. Fahlbush (1999) ArticleTitleBiochemical characterisation of human pituitary somatotrophinomas with and without gsp mutations: in vitro cells culture studies J Clin Endocrinol Metab 80 2077–2081 Occurrence Handle10.1210/jc.80.7.2077

    Article  Google Scholar 

  21. EF Adams T Todo UM Schrell P Thierauf MC White R. Fahlbusch (1999) ArticleTitleAutocrine control of human meningioma proliferation: secretion of platelet-derived growth-factor-like molecules Int J Cancer 3 398–402

    Google Scholar 

  22. CK Goldman J Kim WL Wong V King T Brock GY. Gillespie (1999) ArticleTitleEpidermal growth factor stimulates vascular endothelial growth factor production by human malignant glioma cells: a model of glioblastoma multiforme pathophysiology Mol Biol Cell 1 121–133

    Google Scholar 

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Correspondence to Eric F. Adams.

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Oikonomou, E., Machado, A.L., Buchfelder, M. et al. Meningiomas expressing and responding to cholecystokinin (CCK). J Neurooncol 73, 199–204 (2005). https://doi.org/10.1007/s11060-004-5177-2

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  • DOI: https://doi.org/10.1007/s11060-004-5177-2

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